• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Major royal jelly protein 3 modulates immune responses in vitro and in vivo.
【24h】

Major royal jelly protein 3 modulates immune responses in vitro and in vivo.

机译：主要的蜂王浆蛋白3在体内和体外调节免疫反应。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have recently shown that royal jelly has potent antiallergic properties in a mouse model of immediate hypersensitivity. However, it is still unclear which components of royal jelly exhibit antiallergic activity. In this study, we have screened for antiallergic factors in royal jelly based on inhibition of IL-4 production by anti-CD3 stimulated spleen cells derived from OVA/alum-immunized mice. Using a series of column chromatographies, we purified a 70 kDa glycoprotein, major royal jelly protein 3 (MRJP3), that suppresses IL-4 production. In in vitro experiments, MRJP3 suppressed the production of not only IL-4 but also that of IL-2 and IFN-gamma by T cells concomitant with inhibition of proliferation. The MRJP3-mediated suppression of IL-4 production was also evident when lymph node cells from OVA/alum-immunized mice were stimulated with OVA plus antigen presenting cells. We next examined the purified suppressive factor on OVA/alum-induced allergic responses in mice. Interestingly, in spite of the antigenicity of MRJP3 itself as an extraneous foreign protein, intraperitoneal administration of MRJP3 inhibited serum anti-OVA IgE and IgG1 levels in immunized mice. In addition, heat-treated soluble MRJP3 treatment reduced its antigenicity while maintaining its inhibitory effects on antibody responses to OVA. These results indicate that MRJP3 can exhibit potent immunoregulatory effects in vitro and in vivo. Furthermore, considering the intriguing immunomodulatory effects of MRJP3, it may be of clinical significance to design MRJP3-derived antiallergic peptides by identifying the associated polypeptide regions.
机译：我们最近显示，蜂王浆在立即过敏的小鼠模型中具有有效的抗过敏特性。但是，尚不清楚蜂王浆的哪些成分具有抗过敏活性。在这项研究中，我们已经通过对来自OVA /经alum免疫的小鼠的抗CD3刺激的脾细胞对IL-4产生的抑制作用来筛选蜂王浆中的抗过敏因素。使用一系列柱色谱，我们纯化了一个70 kDa的糖蛋白，主要的蜂王浆蛋白3（MRJP3），可抑制IL-4的产生。在体外实验中，MRJP3不仅抑制T细胞同时抑制增殖的IL-4和IL-2和IFN-γ的产生。当用OVA加抗原呈递细胞刺激OVA /经alum免疫的小鼠的淋巴结细胞时，MRJP3介导的IL-4产生的抑制也很明显。接下来，我们检查了对小鼠中OVA /铝诱导的过敏反应的纯化抑制因子。有趣的是，尽管MRJP3本身具有外源性外源蛋白的抗原性，但腹膜内给予MRJP3可以抑制免疫小鼠的血清抗OVA IgE和IgG1水平。此外，热处理的可溶性MRJP3处理降低了其抗原性，同时保持了其对OVA抗体应答的抑制作用。这些结果表明，MRJP3可以在体外和体内表现出有效的免疫调节作用。此外，考虑到MRJP3令人着迷的免疫调节作用，通过鉴定相关的多肽区域设计MRJP3衍生的抗过敏肽具有临床意义。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号