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首页> 外文期刊>Cell biology international. >Expression of cyclin D1 after treatment with doxorubicin in the HL-60 cell line
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Expression of cyclin D1 after treatment with doxorubicin in the HL-60 cell line

机译：阿霉素处理后HL-60细胞中cyclin D1的表达

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Increased levels of cyclin D1 and amplification of CCND1 gene occur in many types of cancers. We have followed the expression of cyclin D1 after treatment with doxorubicin with reference to cell death and other possible therapeutic implications. The effect of the treatment on the cell cycle, survival, intracellular level (flow cytometry), and intracellular localization of cyclin D1 (fluorescence microscopy) and expression of CCND1 (real-time RT-PCR) was investigated in HL-60 cells. An increase in the fluorescence intensity of cyclin D1 occurred after treatment with 0.15 and 0.3 mM doxorubicin. This tendency was confirmed by real-time RT-PCR. Expression of CCND1 in relation to the reference gene PBGD was increased in cells exposed to 0.15mM doxorubicin. Concomitantly, some alterations in the regulation of the G0/G1, S, and G2/M checkpoints occurred, accompanied by changes in the polyploid fraction of the population. This was particularly evident at 0.3 mM doxorubicin, at which concentration the rate of cell death was also clearly higher. In conclusion, depending on the concentration used, alterations in cell death and the number of S, G2/M, and polyploid cells may correspond with cyclin D1 levels. This, in turn, may reflect an important role of the protein as one of the possible survival/point-of-no-return regulators dependent on its concentration, which seems especially plausible in the context of more prominent cell death in the abovementioned fractions of cells.
机译：细胞周期蛋白D1的水平升高和CCND1基因的扩增发生在许多类型的癌症中。关于细胞死亡和其他可能的治疗意义，我们跟踪了阿霉素治疗后细胞周期蛋白D1的表达。在HL-60细胞中研究了该处理对细胞周期，存活率，细胞内水平（流式细胞仪）和细胞周期蛋白D1的细胞内定位（荧光显微镜）和CCND1表达（实时RT-PCR）的影响。在用0.15和0.3mM阿霉素处理后，细胞周期蛋白D1的荧光强度增加。实时RT-PCR证实了这种趋势。在暴露于0.15mM阿霉素的细胞中，与参考基因PBGD相关的CCND1表达增加。随之而来的是，G0 / G1，S和G2 / M检查站的调节发生了一些变化，并伴随着种群多倍体部分的变化。这在0.3 mM阿霉素时尤其明显，在该浓度下，细胞死亡的速率也明显更高。总之，取决于所使用的浓度，细胞死亡的改变以及S，G2 / M和多倍体细胞的数量可能与细胞周期蛋白D1水平相对应。反过来，这可能反映了蛋白质作为依赖于其浓度的可能的存活/非回归点调节剂之一的重要作用，这在上述组分的更明显的细胞死亡的背景下似乎特别合理。细胞。

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