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首页> 外文期刊>Life sciences >Endometrium estradiol receptors type I and type II during early pregnancy of rat
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Endometrium estradiol receptors type I and type II during early pregnancy of rat

机译：大鼠早期妊娠期间子宫内膜雌二醇受体I型和II型

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摘要

By centrifugation in a sucrose density gradient we studied the citosol 17 beta-estradiol binding sites of blastocyst receptive and non-receptive endometrial zones, as well as uterine horn endometrium whose ovary was extirpated three weeks before pregnancy. The cytosol was prelabelled with {H-3}-17 beta-estradiol 2 and 25 nM. In this work two incubation temperatures were studied. On the other hand, at 4 degrees C unoccupied receptors were identified as different from the classic receptor 8S type I. At the same time, we found that 25 degrees C is the optimal temperature for the assay of total receptors to achieve complete exchange of {H-3}-17 beta-estradiol by 17 beta-estradiol in the binding sites. In these conditions, the major component was the 4S type II receptor, mainly in the endometrium from ovariectomized uteri. Furthermore, 17 beta-estradiol content was determined in the total homogenized by radioimmunoassay and the results were: 1.42 +/- 0.16, 1.22 +/- 0.15 and 1.75 +/- 0.27 pmol/g wet tissue for receptive, non-receptive and ovariectornized uteri, respectively. (c) 2005 Elsevier Inc. All rights reserved.
机译：通过在蔗糖密度梯度中离心，我们研究了囊胚接受性和非接受性子宫内膜区以及子宫角子宫内膜的citosol 17β-雌二醇结合位点，子宫角内膜在怀孕前三周已被切除。用{H-3} -17β-雌二醇2和25 nM预标记细胞质。在这项工作中，研究了两个孵育温度。另一方面，在4°C时，未占用的受体被确定为不同于I型经典受体8S。同时，我们发现25°C是测定总受体以完全交换{ H-3} -17β-雌二醇与17β-雌二醇的结合位点。在这些情况下，主要成分是4S II型受体，主要在卵巢切除子宫的子宫内膜中。此外，在放射免疫分析中均质化的总样品中测定了17种β-雌二醇的含量，结果是：对于接受，不接受和去卵巢的组织，湿组织为1.42 +/- 0.16、1.22 +/- 0.15和1.75 +/- 0.27 pmol / g。子宫。 （c）2005 Elsevier Inc.保留所有权利。

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