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首页> 外文期刊>Life sciences >Effect of an enriched cholesterol diet during gestation on fatty acid synthase, HMG-CoA reductase and SREBP-1/2 expressions in rabbits.
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Effect of an enriched cholesterol diet during gestation on fatty acid synthase, HMG-CoA reductase and SREBP-1/2 expressions in rabbits.

机译：妊娠期间富含胆固醇的饮食对家兔脂肪酸合成酶，HMG-CoA还原酶和SREBP-1 / 2表达的影响。

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摘要

Pregnancy is associated with hyperlipidemia and hypercholesterolemia in humans. These changes take place to support fetal growth and development, and modifications of these maternal concentrations may influence lipids and cholesterol synthesis in the dam, fetus and placenta. Administration of a 0.2% enriched cholesterol diet (ECD) during rabbit gestation significantly increased cholesterol and triglyceride (TG) levels in maternal livers and decreased fetal weight by 15%. Here we used Western blot analysis to examine the impact of gestation and 0.2% ECD on the expression levels of fatty acid synthase (FAS), HMGR and SREBP-1/2, which are involved in either lipid or cholesterol synthesis. We confirmed that gestation modifies the hepatic and circulating lipid profile in the mother. Our data also suggest that the maternal liver mainly supports lipogenesis, while the placenta plays a key role in cholesterol synthesis. Thus, our data demonstrate a decrease in HMGR protein levels in dam livers by feeding an ECD. In the placenta, SREBPs are highly expressed, and the ECD supplementation increased nuclear SREBP-1/2 protein levels. In addition, our results show a decrease in FAS protein levels in non-pregnant liver and in the liver of offspring from ECD-treated animals. Finally, our data suggest that the placenta does not modify its own cholesterol synthesis in response to an increase in circulating cholesterol. However, the dam liver compensates for this increase by essentially decreasing the level of HMGR expression. Because HMGR and FAS expressions do not correlate with the circulating lipid profile, it would be interesting to find which genes are then targeted by SREBP-1/2 during gestation.
机译：怀孕与人类的高脂血症和高胆固醇血症有关。这些变化的发生是为了支持胎儿的生长和发育，而这些母体浓度的改变可能会影响大坝，胎儿和胎盘中的脂质和胆固醇合成。在兔子妊娠期间给予0.2％的富胆固醇饮食（ECD）可以显着提高孕妇肝脏中的胆固醇和甘油三酸酯（TG）水平，并使胎儿体重降低15％。在这里，我们使用蛋白质印迹分析来检查妊娠和0.2％ECD对参与脂质或胆固醇合成的脂肪酸合酶（FAS），HMGR和SREBP-1 / 2表达水平的影响。我们证实，妊娠会改变母亲的肝脏和循环脂质分布。我们的数据还表明，母体肝脏主要支持脂肪生成，而胎盘在胆固醇合成中起关键作用。因此，我们的数据表明通过饲喂ECD可使大坝肝脏中HMGR蛋白水平降低。在胎盘中，SREBPs高度表达，ECD补充增加了核SREBP-1 / 2蛋白的水平。此外，我们的结果表明，未怀孕的肝脏和接受ECD处理的动物的后代肝脏中FAS蛋白水平降低。最后，我们的数据表明，胎盘不会响应循环胆固醇的增加而改变自身的胆固醇合成。但是，大坝肝脏通过基本降低HMGR表达水平来补偿这种增加。因为HMGR和FAS表达与循环脂质谱不相关，所以有趣的是发现妊娠期间SREBP-1 / 2靶向哪些基因。

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