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首页> 外文期刊>Cell biology international. >Effect of phenol red and steroid hormones on cystic fibrosis transmembrane conductance regulator in mouse endometrial epithelial cells.
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Effect of phenol red and steroid hormones on cystic fibrosis transmembrane conductance regulator in mouse endometrial epithelial cells.

机译：酚红和类固醇激素对小鼠子宫内膜上皮细胞囊性纤维化跨膜电导调节剂的影响。

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摘要

Previous studies have demonstrated that cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-mediated Cl(-)channel found in most epithelia including reproductive tract, could be regulated by various culture conditions. The present study further investigated the effect of phenol red, a pH indicator widely used in growth medium, and steroid hormones, present in the supplement fetal bovine serum (FBS), on primary cultured endometrial epithelial cells by monitoring ion channel activities using the short-circuit current technique. When compared to the results obtained with normal medium supplemented with regular FBS, the forskolin-stimulated I(SC), presumably mediated by CFTR, obtained in phenol red-free medium was significantly reduced, from 16.95+/-1.53 microA/cm(2)(control) to 9.72+/-0.89 microA/cm(2)(medium without phenol red, P< 0.05). The forskolin-activated I(SC)was further attenuated to 5.29+/-0.46 microA/cm(2)in the phenol red-free medium when supplemented with charcoal/ dextran-treated FBS where steroid hormones were removed. Our data suggest that phenol red and steroid hormones present in culture medium and FBS supplement, respectively, may somehow upregulate CFTR expression in vitro. Our study demonstrates the need for carefully choosing the culture media and supplements due to the effect of steroid hormones.
机译：以前的研究表明，囊性纤维化跨膜电导调节剂（CFTR）是在大多数上皮包括生殖道中发现的cAMP介导的Cl（-）通道，可以通过各种培养条件来调节。本研究进一步研究了酚红（一种在生长培养基中广泛使用的pH指示剂）和补充胎牛血清（FBS）中存在的类固醇激素对原代培养的子宫内膜上皮细胞的影响，方法是使用短波监测电路电流技术。与使用常规FBS补充的普通培养基所获得的结果相比，在无酚红培养基中获得的大概受CFTR介导的福司柯林刺激的I（SC）从16.95 +/- 1.53 microA / cm（2）显着降低）（对照）至9.72 +/- 0.89 microA / cm（2）（不含酚红的介质，P <0.05）。当添加木炭/右旋糖酐处理过的FBS去除类固醇激素后，在不含酚红的培养基中，福司柯林活化的I（SC）进一步衰减至5.29 +/- 0.46 microA / cm（2）。我们的数据表明，分别存在于培养基和FBS补充物中的酚红和类固醇激素可能以某种方式上调了体外CFTR的表达。我们的研究表明，由于类固醇激素的作用，需要仔细选择培养基和补充剂。

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