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首页> 外文期刊>Life sciences >Cell surface NADH oxidase activity of brine shrimp oscillates with a period of 25 min and is entrained by light.
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Cell surface NADH oxidase activity of brine shrimp oscillates with a period of 25 min and is entrained by light.

机译：盐水虾的细胞表面NADH氧化酶活性以25分钟的周期振荡并被光夹带。

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摘要

Plants have a surface NADH oxidase that measures time by oscillating with a 24-min period. The period is synchronized by light. With plants, a new maximum is observed exactly 12 min after the beginning of the light exposure. These experiments were to determine if animals exhibited a cell surface NADH oxidase having a similar periodicity and to answer the question, does the periodicity in animals respond to light? Using brine shrimp as a model, the findings show that plants and animals exhibit similar oscillating NADH oxidase activity and that the periodicity in this invertebrate animal does respond to light. Brine shrimp were grown for two to three days and transferred to darkness for 45 min. After return to light for one min, NADH was added and measurements of NADH oxidation were recorded over 50 min. The brine shrimp exhibited a cell surface NADH oxidase that oscillated with a period of 25 min. After being subjected to light, the brine shrimp showed a new maximum in NADH oxidation between 12 to 13 min after the beginning of the light exposure and again at 37 min and at 25 min intervals thereafter. The findings demonstrate that the periodic oscillations in NADH oxidation of brine shrimp are light entrainable.
机译：植物具有一个表面NADH氧化酶，该酶通过每24分钟振荡来测量时间。该周期通过光同步。对于植物，在光照开始后恰好12分钟观察到一个新的最大值。这些实验是为了确定动物是否表现出具有相似周期性的细胞表面NADH氧化酶，并回答这个问题，动物的周期性对光有反应吗？以盐水虾为模型，研究结果表明动植物表现出类似的振荡NADH氧化酶活性，并且这种无脊椎动物的周期性确实对光有反应。将盐水虾生长两到三天，然后转移到黑暗中45分钟。放光1分钟后，添加NADH，并记录50分钟内NADH氧化的测量值。盐水虾表现出细胞表面NADH氧化酶的振荡时间为25分钟。光照后，盐水虾在开始曝光后的12至13分钟之间以及之后的37分钟和之后的25分钟间隔内显示出新的NADH氧化最大值。这些发现表明盐水虾的NADH氧化中的周期性振荡是轻度诱人的。

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