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Hyperthermia and tumour necrosis factor-alpha induced apoptosis via mitochondrial damage

机译：热疗和肿瘤坏死因子-α通过线粒体损伤诱导细胞凋亡

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Hyperthermia is a potential anti-cancer regimen but the mode of action is far from clear. Based on the flow cytometric analysis with FITC-annexin V and propidium iodide, apoptosis was found to be the major form of cell death after the treatment with hyperthermia (43 degrees C, 3h) and/or recombinant murine tumour necrosis factor-alpha (TNF-alpha, 50ng/ml) in L929 cells. Since mitochondria are thought to play a key role in apoptosis, experiments were done to assess their role in the hyperthermia-mediated apoptosis. Our results indicate that hyperthermia was able to depolarize the mitochondrial membrane potential (Delta Psi m) and release cytochrome c to the cytoplasm, in a way very similar to the action of TNF-alpha. With the use of cyclosporin A to inhibit the Delta Psi m dissipation, the cytotoxicity mediated by hyperthermia or TNF-alpha was suppressed. Taken together, our results indicate that hyperthermia and TNF-alpha can induce apoptosis in L929 cells and the mitochondrial dysfunction plays a key role in the cell death process. (C) 2000 Elsevier Science Inc. All rights reserved. [References: 18]
机译：热疗是一种潜在的抗癌方案，但作用方式尚不清楚。基于FITC-annexin V和碘化丙啶的流式细胞仪分析，发现凋亡是高温（43摄氏度，3小时）和/或重组鼠肿瘤坏死因子-α（TNF）治疗后细胞死亡的主要形式。 -α，50ng / ml）在L929细胞中。由于线粒体被认为在细胞凋亡中起关键作用，因此进行了实验以评估其在高温介导的细胞凋亡中的作用。我们的结果表明，热疗能够使线粒体膜电位去极化（Delta Psi m），并将细胞色素c释放到细胞质中，其方式与TNF-α的作用非常相似。通过使用环孢菌素A抑制Delta Psi m耗散，抑制了由高温或TNF-α介导的细胞毒性。两者合计，我们的结果表明，高温和TNF-α可以诱导L929细胞凋亡，而线粒体功能障碍在细胞死亡过程中起关键作用。 （C）2000 Elsevier Science Inc.保留所有权利。 [参考：18]

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