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首页> 外文期刊>Life sciences >Hyperglycemia in diabetic rats reduces the glutathione content in the aortic tissue.
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Hyperglycemia in diabetic rats reduces the glutathione content in the aortic tissue.

机译：糖尿病大鼠中的高血糖会降低主动脉组织中的谷胱甘肽含量。

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The glutathione redox cycle plays a major role in scavenging hydrogen peroxide (H2O2) under physiological conditions. Recently, we demonstrated that a high glucose concentration in the culture medium reduced the level of H2O2 scavenging activity of human vascular smooth muscle cells (hVSMCs). We also showed that a high glucose concentration reduced the intracellular glutathione (GSH) content and the rate of uptake of cystine, which itself is a rate-limiting factor that maintains the GSH level (FEBS Lett.421: 19-22,1998). In the present study, we investigated whether the hyperglycemic condition in diabetic rats impairs the glutathione content in the aortic tissue in vivo. Wistar rats were divided into the following three groups: streptozotocin-induced diabetic rats (STZ-D, n=7), insulin-treated STZ-D rats (I-STZ-D, n=8), and non-diabetic controls (C, n=7). Fourteen days after streptozotocin injection, the aortic tissue was extracted and the GSH content in the aortic tissue was measured. Furthermore, the relationship between the GSH content in the aortic tissue and blood glucose level in Otsuka Long-Evans Tokushima Fatty (OLETF) rats aged 30 weeks, which developed diabetes spontaneously, was investigated. The GSH content in the aortic tissue of the STZ-D group (0.99+/-0.14 nmol/mg protein) was significantly lower than that of the control group (1.68+/-0.15 nmol/mg protein). Insulin treatment to the diabetic rats restored the GSH content in the aortic tissue (I-STZ-D group; 1.45+/-0.11 nmol/mg protein). Among the 22 Wistar rats, the GSH content in the aortic tissue was negatively correlated with the blood glucose level (r=-0.69, p<0.01, n=22). Among the OLETF rats, a similar negative correlation between the GSH content in the aortic tissue and blood glucose level was seen (r=-0.64, p<0.05, n=10). We demonstrated in vivo that the hyperglycemic condition in STZ-induced diabetic Wistar rats and OLETF rats reduced the GSH content in aortic tissue. This suggested reduced glutathione redox cycle function of aorta.
机译：在生理条件下，谷胱甘肽氧化还原循环在清除过氧化氢（H2O2）中起主要作用。最近，我们证明了培养基中的高葡萄糖浓度降低了人类血管平滑肌细胞（hVSMCs）对H2O2的清除活性水平。我们还表明，高葡萄糖浓度会降低细胞内谷胱甘肽（GSH）含量和胱氨酸的摄取速率，胱氨酸本身是维持GSH水平的限速因子（FEBS Lett.421：19-22，1998）。在本研究中，我们调查了糖尿病大鼠的高血糖状况是否会损害体内主动脉组织中的谷胱甘肽含量。 Wistar大鼠分为以下三组：链脲佐菌素诱导的糖尿病大鼠（STZ-D，n = 7），胰岛素治疗的STZ-D大鼠（I-STZ-D，n = 8）和非糖尿病对照组（ C，n ＝ 7）。注射链脲佐菌素后第14天，提取主动脉组织并测量主动脉组织中的GSH含量。此外，研究了自发性糖尿病的30周大冢大埃文斯德岛肥胖（OLETF）大鼠的主动脉组织中GSH含量与血糖水平之间的关系。 STZ-D组主动脉组织中的GSH含量（0.99 +/- 0.14 nmol / mg蛋白）显着低于对照组（1.68 +/- 0.15 nmol / mg蛋白）。对糖尿病大鼠的胰岛素治疗恢复了主动脉组织（I-STZ-D组; 1.45 +/- 0.11 nmol / mg蛋白）中的GSH含量。在22只Wistar大鼠中，主动脉组织中GSH含量与血糖水平呈负相关（r = -0.69，p <0.01，n = 22）。在OLETF大鼠中，主动脉组织中GSH含量与血糖水平之间存在相似的负相关（r = -0.64，p <0.05，n = 10）。我们在体内证明了STZ诱导的糖尿病Wistar大鼠和OLETF大鼠的高血糖状况降低了主动脉组织中的GSH含量。这表明主动脉的谷胱甘肽氧化还原循环功能降低。

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