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首页> 外文期刊>Life sciences >Synergy between ascorbate and alpha-tocopherol on fibroblasts in culture.
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Synergy between ascorbate and alpha-tocopherol on fibroblasts in culture.

机译：培养物中成纤维细胞上抗坏血酸盐和α-生育酚之间的协同作用。

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摘要

Ascorbate and tocopherol are important antioxidants that protect cells against oxidative stress. The interaction of ascorbate and alpha-tocopherol in cells is difficult to detect as both ascorbate and alpha-tocopherol are unstable in vitro in a biological medium. We examined the interactions between human dermal fibroblasts, ascorbate and alpha-tocopherol to determine the effects of the vitamins on growth and cell viability. The interaction of ascorbate and alpha-tocopherol was studied in a fibroblast culture medium during 48h. Ascorbate and alpha-tocopherol were detected by fluorimetry after high-performance liquid chromatography (HPLC). Cell growth and cell viability were studied by cell numeration after trypan blue staining. The ascorbate concentration fell in presence of alpha-tocopherol in cell culture medium under all experimental conditions, with or without cells. Ascorbate partly protected alpha-tocopherol but only in presence of cells. Cell viability was preserved by alpha-tocopherol whereas ascorbate enhanced fibroblast growth. The synergy between ascorbate and alpha-tocopherol corresponds to a consumption of ascorbate which spares alpha-tocopherol but only in presence of cells.
机译：抗坏血酸和生育酚是保护细胞免受氧化应激的重要抗氧化剂。细胞中抗坏血酸和α-生育酚的相互作用很难检测，因为抗坏血酸和α-生育酚在体外在生物培养基中都是不稳定的。我们检查了人类皮肤成纤维细胞，抗坏血酸和α-生育酚之间的相互作用，以确定维生素对生长和细胞活力的影响。在成纤维细胞培养基中研究了抗坏血酸和α-生育酚的相互作用，历时48h。高效液相色谱（HPLC）后，通过荧光法检测抗坏血酸和α-生育酚。台盼蓝染色后通过细胞计数研究细胞生长和细胞活力。在有或没有细胞的所有实验条件下，在细胞培养基中存在α-生育酚的情况下，抗坏血酸的浓度都会下降。抗坏血酸部分保护α-生育酚，但仅在存在细胞的情况下。 α-生育酚可保留细胞活力，而抗坏血酸可增强成纤维细胞生长。抗坏血酸盐和α-生育酚之间的协同作用对应于抗坏血酸盐的消耗，其消耗α-生育酚但仅在存在细胞的情况下。

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