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Preferential expression of ADP-ribosylation factor gene in the chick embryonic gonads

机译:ADP-核糖基化因子基因在雏鸡性腺中的优先表达

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cDNA cloning from chick embryonic gonads subtracted from tissues of the brain, heart, liver, gizzard, mesonephros and skeletal muscle was performed to identify genes with expression unique to embryonic gonads. Several cDNA clones encoding characterized as well as many uncharacterized genes were obtained. ADP-ribosylation factor (ARF) of these identified genes was preferentially expressed in the chick embryonic ovary and testis as revealed by reverse transcription-polymerase chain reaction analysis. Expression of the ARF was evaluated through embryonic development, but no difference in the transcript (relative to glyceraldehyde-3-phosphate dehydrogenase transcript) was observed between the left and right ovaries, and between the ovary and testis. In addition, the ART transcript was detected in the gonads on embryonic days 5 to 21. These findings indicate that the ARF is constantly, but preferentially expressed in the embryonic gonads during development. (C) 2002 Elsevier Science Inc. All rights reserved. [References: 18]
机译:进行了从脑,心脏,肝脏,g,中肾和骨骼肌组织中减去的鸡胚性腺的cDNA克隆,以鉴定具有胚性腺特有表达的基因。获得了几个编码特征性的cDNA克隆以及许多未表征的基因。如逆转录-聚合酶链反应分析所揭示,这些鉴定出的基因的ADP-核糖基化因子(ARF)优先在雏鸡的卵巢和睾丸中表达。通过胚胎发育评估了ARF的表达,但是在左右卵巢之间以及卵巢和睾丸之间没有观察到转录本的差异(相对于3-磷酸甘油醛脱氢酶的转录本)。另外,在胚胎第5至21天在性腺中检测到ART转录物。这些发现表明,ARF在发育过程中在胚胎性腺中不断表达,但优先表达。 (C)2002 Elsevier Science Inc.保留所有权利。 [参考:18]

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