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首页> 外文期刊>Life sciences >Exposure to the environmental estrogen bisphenol A differentially modulated estrogen receptor-alpha and -beta immunoreactivity and mRNA in male mouse testis.
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Exposure to the environmental estrogen bisphenol A differentially modulated estrogen receptor-alpha and -beta immunoreactivity and mRNA in male mouse testis.

机译：暴露于雄性小鼠睾丸中环境雌激素双酚A差异调节的雌激素受体α和β免疫反应性和mRNA。

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摘要

We examined the effects of bisphenol A (0.5 microg/ml or 50 microg/ml) in the drinking water on estrogen receptor (ER) alpha and beta proteins and mRNA in the testis of young mice following 8-weeks of oral administration of bisphenol A utilizing immunohistochemistry and semiquantitative reverse transcription polymerase chain reaction amplification (RT-PCR). ER beta was clearly localized in the nuclei of spermatogonia and/or spermatocytes. ER beta immunopositive cell numbers per testis section were significantly decreased in the 50 microg/ml bisphenol A-treated group compared with control and the 0.5 microg/ml bisphenol A-treated group. The number of ER alpha positive cells in the testis was significantly lower than ER beta positive cells in control group. ER alpha immunopositive cell numbers per testis section were markedly increased in the 50 microg/ml bisphenol A-treated group compared with the control and the 0.5 microg/ml bisphenol A-treated group. ER beta mRNA expression was significantly decreased in the 50 microg/ml bisphenol A-treated group compared with the control and the 0.5 microg/ml bisphenol A-treated group. In contrast, ER alpha mRNA expression was markedly increased in the 50 microg/ml bisphenol A-treated group compared with the control and the 0.5 microg/ml bisphenol A-treated group. The existence of ER alpha and beta in the testis suggests that estrogens directly affect germ cells during testicular development and spermatogenesis, and differential modulation of ER alpha and beta in the testis could be involved in the effects of bisphenol A.
机译：我们研究了双酚A口服8周后，饮用水中双酚A（0.5微克/毫升或50微克/毫升）对年轻小鼠睾丸中雌激素受体（ER）α和β蛋白和mRNA的影响利用免疫组织化学和半定量逆转录聚合酶链反应扩增（RT-PCR）。 ER beta明显位于精原细胞和/或精母细胞核中。与对照组和0.5微克/毫升双酚A处理组相比，在50微克/毫升双酚A处理组中，每个睾丸切片的ERβ免疫阳性细胞数量明显减少。睾丸中的ERα阳性细胞数量明显低于对照组的ERβ阳性细胞。与对照组和0.5微克/毫升双酚A处理组相比，在50微克/毫升双酚A处理组中，每个睾丸切片的ERα免疫阳性细胞数明显增加。与对照组和0.5微克/毫升双酚A处理组相比，在50微克/毫升双酚A处理组中ERβmRNA表达显着降低。相反，与对照组和0.5μg/ ml双酚A处理组相比，在50μg/ ml双酚A处理组中ERαmRNA表达显着增加。睾丸中ERα和β的存在表明，雌激素直接影响睾丸发育和生精过程中的生殖细胞，而睾丸中ERα和β的差异调节可能与双酚A的作用有关。

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