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首页> 外文期刊>Cell and Tissue Research >Expression of the clock genes Per1 and Bmal1 during follicle development in the rat ovary. Effects of gonadotropin stimulation and hypophysectomy
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Expression of the clock genes Per1 and Bmal1 during follicle development in the rat ovary. Effects of gonadotropin stimulation and hypophysectomy

机译：大鼠卵巢卵泡发育过程中时钟基因Per1和Bmal1的表达。促性腺激素刺激和垂体切除的效果

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摘要

Daily oscillations of clock genes have recently been demonstrated in the ovaries of several species. Clock gene knockout or mutant mice demonstrate a variety of reproductive defects. Accumulating evidence suggests that these rhythms act to synchronise the expression of specific ovarian genes to hypothalamo-pituitary signals and that they are regulated by one or both of the gonadotropins. The aim of this study has been to examine the spatio-temporal expression of the clock genes Per1 and Bmal1 during gonadotropin-independent and gonadotropin-dependent follicle development in the rat ovary. We have examined the ovaries of prepubertal rats, of prepubertal rats stimulated with equine chorionic gonadotropin (eCG)/human chorionic gonadotropin (hCG) and of hypophysectomised adult animals. Using quantitative reverse transcription with the polymerase chain reaction, in situ hybridisation histochemistry and immunohistochemistry, we have demonstrated that the expression of the two clock genes is low and arrhythmic in ovarian cells during early gonadotropin- independent follicle development in prepubertal animals and in hypophysectomised animals. We have also demonstrated that the expression of the clock genes becomes rhythmic following eCG stimulation in the theca interna cells and the secondary interstitial cells and that, following additional hCG stimulation, the expression of the clock genes also becomes rhythmic in the granulosa cells of preovulatory follicles. These findings link the initiation of clock gene rhythms in the rat ovary to the luteinising hormone receptor and suggest a functional link to androgen and progesterone production. In hypophysectomised animals, rhythmic clock gene expression is also observed in the corpora lutea and in secondary interstitial cells demonstrating that, in these compartments, entrainment of clock gene rhythms is gonadotropinindependent.
机译：最近已经在几种物种的卵巢中证明了时钟基因的每日振荡。 Clock基因敲除或突变小鼠表现出多种生殖缺陷。越来越多的证据表明，这些节律的作用是使特定卵巢基因的表达与下丘脑-垂体信号同步，并且它们受一种或两种促性腺激素调节。这项研究的目的是检查大鼠卵巢中促性腺激素非依赖性和促性腺激素依赖性卵泡发育过程中时钟基因Per1和Bmal1的时空表达。我们已经检查了青春期前大鼠的卵巢，用马绒毛膜促性腺激素（eCG）/人绒毛膜促性腺激素（hCG）刺激的青春期前大鼠和垂体切除的成年动物的卵巢。使用定量逆转录与聚合酶链反应，原位杂交组织化学和免疫组织化学方法，我们证明了在青春期前动物和垂体切除术动物的早期促性腺激素非依赖性卵泡发育过程中，卵巢细胞中两个时钟基因的表达较低且心律不齐。我们还证明了时钟基因的表达在卵泡膜间质细胞和次级间质细胞中受到eCG刺激后有节律，并且，在额外的hCG刺激后，时钟基因在排卵前卵泡的颗粒细胞中也有节律地表达。这些发现将大鼠卵巢中时钟基因节律的启动与黄体生成激素受体联系起来，并暗示了与雄激素和孕激素产生的功能联系。在垂体切除的动物中，在黄体和次级间质细胞中也观察到节律性时钟基因表达，这表明在这些隔室中，时钟基因节律的夹带是促性腺激素依赖性的。

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