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首页> 外文期刊>Cell and Tissue Research >Fibronectin promotes migration, alignment and fusion in an in vitro myoblast cell model.
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Fibronectin promotes migration, alignment and fusion in an in vitro myoblast cell model.

机译：纤连蛋白在体外成肌细胞模型中促进迁移，对齐和融合。

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摘要

Myogenesis is a complex process in which committed myogenic cells differentiate and fuse into myotubes that mature into the muscle fibres of adult organisms. This process is initiated by a cascade of myogenic regulatory factors expressed upon entry of the cells into the myogenic differentiation programme. However, external signals such as those provided by the extracellular matrix (ECM) are also important in regulating muscle differentiation and morphogenesis. In the present work, we have addressed the role of various ECM substrata on C2C12 myoblast behaviour in vitro. Cells grown on fibronectin align and fuse earlier than cells on laminin or gelatine. Live imaging of C2C12 myoblasts on fibronectin versus gelatine has revealed that fibronectin promotes a directional collective migratory behaviour favouring cell-cell alignment and fusion. We further demonstrate that this effect of fibronectin is mediated by RGD-binding integrins expressed on myoblasts, that N-cadherin contributes to this behaviour, and that it does not involve enhanced myogenic differentiation. Therefore, we suggest that the collective migration and alignment of cells seen on fibronectin leads to a more predictable movement and a positioning that facilitates subsequent fusion of myoblasts. This study highlights the importance of addressing the role of fibronectin, an abundant component of the interstitial ECM during embryogenesis and tissue repair, in the context of myogenesis and muscle regeneration.
机译：肌发生是一个复杂的过程，其中定型的成肌细胞分化并融合成成熟于成年生物的肌纤维的肌管。该过程由细胞进入成肌分化程序后表达的一连串成肌调控因子启动。但是，诸如细胞外基质（ECM）提供的外部信号在调节肌肉分化和形态发生中也很重要。在目前的工作中，我们已经解决了各种ECM基质在体外对C2C12成肌细胞行为的作用。在纤连蛋白上生长的细胞比层粘连蛋白或明胶上的细胞更早排列和融合。在纤连蛋白与明胶上对C2C12成肌细胞进行的实时成像显示，纤连蛋白可促进定向的集体迁徙行为，有利于细胞与细胞的排列和融合。我们进一步证明纤连蛋白的这种作用是由成肌细胞上表达的RGD结合整联蛋白介导的，N-钙粘着蛋白有助于这种行为，并且它不涉及增强的成肌分化。因此，我们建议在纤连蛋白上看到的细胞的集体迁移和排列导致更可预测的运动和定位，有利于成肌细胞的后续融合。这项研究强调了在肌生成和肌肉再生的背景下，解决纤连蛋白（间质ECM在胚胎发生和组织修复过程中的丰富成分）的作用的重要性。

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