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首页> 外文期刊>Cell and Tissue Research >Potential of 2D crosslinked sericin membranes with improved biostability for skin tissue engineering
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Potential of 2D crosslinked sericin membranes with improved biostability for skin tissue engineering

机译：具有改善的生物稳定性的2D交联丝胶蛋白膜在皮肤组织工程中的潜力

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Silk sericin protein is a natural, hydrophilic, macromolecular glycoprotein mainly synthesized in the middle silk gland of the silkworm. It constitutes 25-30% of the silk cocoon. Sericin proteins have antioxidant, antimicrobial, UV-resistant properties, promote wound healing and support cell proliferation even in serum-free media. Most of the sericin is discarded as waste in silk processing industries. This study aims at improving the mechanical strength and stability of sericin extracted from the silk cocoons during processing and utilize it as a biocompatible natural biopolymer in biomedical applications. Crosslinked sericin membranes, from the cocoon of non-mulberry tropical silkworm, Antheraea mylitta, were prepared using gluteraldehyde as the crosslinking agent. Physical and structural characteristics of the membranes were analyzed using scanning electron microscopy, atomic force microscopy, Fourier transform infrared spectroscopy and X-ray diffraction along with swelling and degradation studies. The secondary structure of the membrane indicates that crosslinking provides a more integrated structure that significantly improves the stability and mechanical strength of the membranes. In vitro cytocompatibility of the membranes was evaluated by MTT assay and cell cycle analysis of feline fibroblast cells. The adherence, growth and proliferation patterns of cells on membranes were assessed by confocal microscopy, which demonstrated that the latter is non-toxic and supports cell growth. Cell cycle analyses indicate cytocompatibility with normal cell cycle pattern. This study reveals that silk sericin protein can be used as a biocompatible natural biopolymer for various applications in the biomedical field.
机译：蚕丝丝胶蛋白是一种天然的亲水性大分子糖蛋白，主要在蚕的中部丝腺中合成。它占蚕茧的25％至30％。丝胶蛋白具有抗氧化，抗微生物，抗紫外线的特性，即使在无血清的培养基中也能促进伤口愈合并支持细胞增殖。大多数丝胶在丝绸加工业中被当作废物丢弃。这项研究旨在提高在加工过程中从蚕茧中提取的丝胶的机械强度和稳定性，并将其用作生物医学应用中的生物相容性天然生物聚合物。使用戊二醛作为交联剂，从非桑蚕热带蚕茧中提取了交联的丝胶蛋白膜。膜的物理和结构特征使用扫描电子显微镜，原子力显微镜，傅立叶变换红外光谱和X射线衍射以及溶胀和降解研究进行了分析。膜的二级结构表明交联提供了更完整的结构，可显着提高膜的稳定性和机械强度。通过MTT测定和猫成纤维细胞的细胞周期分析评价膜的体外细胞相容性。通过共聚焦显微镜评估细胞在膜上的粘附，生长和增殖模式，这证明后者是无毒的并且支持细胞生长。细胞周期分析表明具有正常细胞周期模式的细胞相容性。这项研究表明，丝胶蛋白可以作为生物相容性天然生物聚合物用于生物医学领域的各种应用。

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