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首页> 外文期刊>Life sciences >Armepavine oxalate induces cell death on CCRF-CEM leukemia cell line through an apoptotic pathway.
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Armepavine oxalate induces cell death on CCRF-CEM leukemia cell line through an apoptotic pathway.

机译：草酸阿米帕生草酸盐通过凋亡途径诱导CCRF-CEM白血病细胞系死亡。

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摘要

Drug-induced cell death can occur as a result of DNA damage, which in turn may lead to the reduction of bcl-2 expression and activation of caspase-3 expression. In the present study, we investigated the effect of armepavines and atherosperminine on the cell survival rate and expression of bcl-2 and caspase-3 in CCRF-CEM cells. Our data have revealed that armepavine oxalate reduced the survival rate of CCRF-CEM cells in a dose- and time-dependent manner by MTT assay. However, no significant effects of armepavine MeI and atherosperminine N-oxide on the survival rate of the CCRF-CEM cell were observed. Armepavine oxalate-induced cell death was considered to be apoptotic on the basis of observed formation of the DNA ladder and the typical apoptotic morphological change by Hoechst 33258 staining. The expression of bcl-2 protein in CCRF-CEM cells treated with 30 microM armepavine oxalate was significantly decreased in western blotting analysis. In contrast, the expression of active caspase-3 in the cells wasincreased by armepavine oxalate in a dose-dependent manner. These findings indicate the involvement of bcl-2 and caspase-3 in the apoptotic process of CCRF-CEM cells induced by armepavine oxalate. The increased expression of active caspase-3 as well as decreased expression of bcl-2 support the assumption the armepavine oxalate-treated cells may be capable to complete the entire apoptotic process ending in cell fragmentation.
机译：DNA损伤可能导致药物诱导的细胞死亡，进而导致bcl-2表达减少和caspase-3表达激活。在本研究中，我们研究了阿卡波威和动脉粥样硬化对CCRF-CEM细胞的细胞存活率以及bcl-2和caspase-3表达的影响。我们的数据显示，MTT分析表明，草酸阿波威草酸盐以剂量和时间依赖性的方式降低了CCRF-CEM细胞的存活率。然而，没有观察到阿卡波韦MeI和动脉粥样硬化N-氧化物对CCRF-CEM细胞存活率的显着影响。根据观察到的DNA梯形的形成和Hoechst 33258染色的典型凋亡形态学改变，将草酸乙肝单抗诱导的细胞死亡视为凋亡。 Western印迹分析显示，用30 microM阿魏酸草甘膦处理的CCRF-CEM细胞中bcl-2蛋白的表达显着降低。相比之下，草酸阿玛帕文增加了细胞中活性caspase-3的表达，且呈剂量依赖性。这些发现表明bcl-2和caspase-3参与了由草酸阿魏酸所诱导的CCRF-CEM细胞的凋亡过程。活性caspase-3的表达增加以及bcl-2的表达减少支持以下假设：经草酸草酸处理的细胞可能能够完成以细胞破碎为结尾的整个凋亡过程。

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