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首页> 外文期刊>Life sciences >The inhibitory effect of a Korean herbal medicine, Zedoariae rhizoma, on growth of cultured human hepatic myofibroblast cells.
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The inhibitory effect of a Korean herbal medicine, Zedoariae rhizoma, on growth of cultured human hepatic myofibroblast cells.

机译：韩国草药根瘤菌对培养的人肝成肌纤维细胞生长的抑制作用。

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The aim of this study was to assess the effect of ZR on the growth of cultured human hepatic myofibroblast cells (hMF). The zedoary (Zedoariae Rhizoma) made from the dried rhizome of Curcuma zedoaria Roscoe is an herbal drug used as an aromatic stomachic. The plant is a perennial herb which is natively distributed throughout Korea and is a traditional Korean herbal medicine. Zedoariae rhizoma is a bioactive traditional medicine with anti-tumor, anti-atherosclerosis, anti-inflammation, and growth-regulating properties. During the course of liver fibrogenesis, hMF, mostly derived from hepatic stellate cells, proliferate and synthesize excessive amounts of extracellular matrix components. To evaluate the antiproliferative effect of a traditional herbal medicine, Zedoariae rhizoma water extracts (ZR) was examined on the growth inhibition of hMF since proliferation of hMF is known to be central for the development of fibrosis during liver injury, and factors that may limit their growth are potential antifibrotic agents. The aim of this study was to test the effects of ZR on the proliferation in cultured hMF. hMF were obtained by outgrowth from human liver explants. ZR markedly reduced serum driven cell proliferation, as assessed by nuclear autoradiography experiments and measurement of actual cell growth. Growth inhibition was totally reversed after removal of the ZR. ZR potently inhibited hMF growth (IC(50)=8.5 mug/ml), in a pertussis toxin-insensitive manner. Analysis of the mechanisms involved in growth inhibition revealed that ZR rapidly increased prostaglandin E2 production and in turn cAMP, which inhibited hMF proliferation, did not affect cAMP levels. Production of cAMP by ZR was abolished by NS-398, a selective inhibitor of cycloxygenase (COX)-2. Also, ZR potently induced COX-2 protein expression. Blocking COX-2 by NS-398 blunted the antiproliferative effect of ZR. We conclude that ZR inhibits proliferation of hMF, probably via an intracellular mechanism, through early COX-2-dependent release of prostaglandin E2 and cAMP, and delayed COX-2 induction. Our results indicated a novel role for ZR as a growth inhibitory mediator and pointed out its potential involvement in the negative regulation of liver fibrogenesis. The results that ZR exhibits potent antiproliferative and antifibrogenic effects toward hMF, indicated that ZR might have therapeutic implications in chronic liver disease.
机译：这项研究的目的是评估ZR对培养的人肝成纤维细胞（hMF）生长的影响。由姜黄zedoaria Roscoe的干燥根茎制成的zedoary（Zedoariae Rhizoma）是一种用作芳香族胃药的草药。该植物是多年生草本植物，在韩国本土分布，是传统的韩国草药。 Zedoariae rhizoma是一种具有生物活性的传统药物，具有抗肿瘤，抗动脉粥样硬化，抗发炎和调节生长的特性。在肝纤维化过程中，主要来源于肝星状细胞的hMF增殖并合成过量的细胞外基质成分。为了评估传统草药的抗增殖作用，研究了莪术水提取物（ZR）对hMF的生长抑制作用，因为已知hMF的增殖是肝损伤过程中纤维化发展的中心，并且限制了它们生长是潜在的抗纤维化剂。这项研究的目的是测试ZR对培养的hMF增殖的影响。 hMF通过人肝外植体的生长获得。 ZR显着降低了血清驱动的细胞增殖，这是通过核放射自显影实验和实际细胞生长测量来评估的。去除ZR后，生长抑制完全逆转。 ZR以对百日咳毒素不敏感的方式有效抑制hMF生长（IC（50）= 8.5杯/毫升）。对参与生长抑制的机制的分析表明，ZR迅速增加了前列腺素E2的产生，反过来抑制hMF增殖的c​​AMP却不影响cAMP的水平。 ZR产生的cAMP被NS-398（一种环氧合酶（COX）-2的选择性抑制剂）废除了。此外，ZR可以有效诱导COX-2蛋白表达。 NS-398阻止COX-2抑制了ZR的抗增殖作用。我们得出的结论是，ZR可能通过细胞内机制，通过早期COX-2依赖的前列腺素E2和cAMP的释放以及延迟的COX-2诱导来抑制hMF的增殖。我们的结果表明ZR作为生长抑制介质的新作用，并指出其潜在参与肝脏纤维发生的负调控。 ZR对hMF表现出有效的抗增殖和抗纤维化作用的结果表明ZR可能对慢性肝病具有治疗意义。

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