• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Molecular dissection of a medicinal herb with anti-tumor activity by oligonucleotide microarray.
【24h】

Molecular dissection of a medicinal herb with anti-tumor activity by oligonucleotide microarray.

机译：通过寡核苷酸微阵列对具有抗肿瘤活性的草药进行分子解剖。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

It is difficult to understand precisely the physiological actions of herbs because they contain a complex array of constituent molecules. In the present study we used DNA microarray data for 12600 genes to examine the anti-proliferative activity of the herb Coptidis rhizoma and eight constituent molecules against eight human pancreatic cancer cell lines. We identified 27 genes showing strong correlation with the 50% inhibitory dose (ID50) of C. rhizoma after 72-h exposure. Hierarchical cluster analysis with correlation coefficients between expression levels of these 27 C. rhizoma-related genes and the ID50 of each constituent molecule classified these test molecules into two clusters, one consisting of C. rhizoma and berberine and the other consisting of the remaining seven molecules. Our results suggest that one molecule, berberine, can account for the majority of the anti-proliferative activity of C. rhizoma and that DNA microarray analyses can be used to improve our understanding of the actions of an intact herb.
机译：很难准确了解草药的生理作用，因为它们包含复杂的组成分子阵列。在本研究中，我们使用了12600个基因的DNA微阵列数据来检查草药黄连和八个组成分子对八种人类胰腺癌细胞系的抗增殖活性。我们鉴定了27个基因，与暴露后72小时的50％根瘤菌抑制剂量（ID50）密切相关。层次聚类分析，利用这27个与根瘤菌相关基因的表达水平与每个组成分子的ID50之间的相关系数，将这些测试分子分为两个聚类，一个由根瘤菌和小ber碱组成，另一个由其余七个分子组成。我们的结果表明，一种分子小ber碱可以构成根瘤菌的大部分抗增殖活性，并且可以使用DNA微阵列分析来增进我们对完整草药作用的了解。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号