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首页> 外文期刊>Life sciences >Multiple calmodulin mRNAs are selectively transported to functionally different neuronal and glial compartments in the rat hippocampus. An electron microscopic in situ hybridization study.
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Multiple calmodulin mRNAs are selectively transported to functionally different neuronal and glial compartments in the rat hippocampus. An electron microscopic in situ hybridization study.

机译：多种钙调蛋白mRNA被选择性转运至大鼠海马中功能不同的神经元和神经胶质区室。电子显微镜原位杂交研究。

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摘要

The ultrastructural distribution of the calmodulin (CaM) mRNAs transcribed from the three CaM genes was studied in the CA1 region of the adult rat hippocampus by means of electron microscopic in situ hybridization. Digoxigenin-labeled CaM gene-specific riboprobes were detected with nanogold-anti-digoxigenin antibody conjugate. The CaM mRNAs were differentially distributed in both the neuronal and glial cell compartments. The greatest difference in neuronal distribution of the CaM mRNAs was found in the dendrites, where the mRNAs transcribed from the CaM I and III genes were much more abundant than the CaM II mRNA. The neuronal perikarya were heavy labeled for all the CaM mRNAs. Interestingly, the myelinated axons and axon terminals also contained small amounts of nanogold particles for all the CaM mRNAs, which diminished with increasing distance from the soma. Most of the synaptic profiles, however, contained labeling only in the postsynaptic region. The CaM mRNAs were differentially distributed in theglial cells. While the glial cell somata were only lightly labeled, surprisingly concentrated labeling was present in the perisynaptic and perivascular astrocytic processes. In general, the CaM II mRNA was the least represented in the glial processes. Only a very low CaM gene expression was observed in the endothelial and resting microglial cells. These results provide ultrastructural evidence for differential targeting of the multiple CaM mRNA transcripts to the intracellular compartments and suggest their microdomain-specific regulation.
机译：通过电子显微镜原位杂交研究了在成年大鼠海马CA1区中从这三个CaM基因转录的钙调蛋白（CaM）mRNA的超微结构分布。用纳米金-抗-地高辛配基抗体结合物检测地高辛配基标记的CaM基因特异性核糖。 CaM mRNA在神经元和神经胶质细胞区室中差异分布。 CaM mRNA的神经元分布的最大差异是在树突中发现的，其中从CaM I和III基因转录的mRNA比CaM II mRNA丰富得多。神经元周围核被重标记了所有的CaM mRNA。有趣的是，髓鞘化的轴突和轴突末端还包含所有CaM mRNA的少量纳米金颗粒，随着距躯体距离的增加而减小。但是，大多数突触图只在突触后区域包含标记。 CaM mRNA在神经胶质细胞中差异分布。虽然仅轻度标记了神经胶质细胞体细胞，但突触周围和血管周围星形胶质细胞过程中却出现了令人惊讶的浓缩标记。通常，CaM II mRNA在神经胶质过程中代表最少。在内皮和静息的小胶质细胞中仅观察到非常低的CaM基因表达。这些结果为将多个CaM mRNA转录物差异靶向到细胞内区室提供了超微结构证据，并表明了它们的微区特异性调控。

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