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首页> 外文期刊>Life sciences >The effect of chronic calcium treatment on thyroid C cells in ovariectomized rats.
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The effect of chronic calcium treatment on thyroid C cells in ovariectomized rats.

机译：慢性钙治疗对去卵巢大鼠甲状腺C细胞的影响。

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The purpose of this study was to investigate the influence of chronic calcium treatment on the structure and function of thyroid C cells in ovariectomzed adult female rats. Eighteen 3-month-old, female Wistar rats were divided into three groups. The first group was used as the sham-operated control, and the other two were surgically ovariectomized (Ovx). One month after gonadectomy, one group of Ovx rats was injected with 28.55 mg Ca-glucoheptonate (Ca)/kg b.w., while the other two groups were chronically treated with vehicle alone (Ovx and sham control). Two months after surgery, the animals were killed. In the thyroid C cells, calcitonin (CT) was localized with the peroxidase-antiperoxidase method. Stereology was used to evaluate morphometric changes in the volume of C cells, their nuclei and relative volume density. The number of C cells per unit area was calculated. Serum CT content was determined by radioimmunoassay. After chronic Ca treatment C cells were numerous with darker cytoplasm than in C cells of sham-operated control animals, but more degranulated than the corresponding cells of Ovx rats. Their volume was significantly decreased by 14% (p < 0.05), while the number was increased by 47% (p < 0.05) in comparison with corresponding controls. Serum CT concentration was decreased by 27% (n.s.) in comparison to sham-operated control. Calcium treatment of Ovx rats led to a 32% increase of serum CT concentration in relation to untreated Ovx animals. These results suggest that chronic Ca treatment of Ovx female rats positively affected CT release from thyroid C cells, without any significant changes in morphometric parameters.
机译：这项研究的目的是调查长期钙治疗对去卵巢成年雌性大鼠甲状腺C细胞的结构和功能的影响。将18只3个月大的雌性Wistar大鼠分为三组。第一组用作假手术对照组，另两组通过手术切除卵巢（Ovx）。性腺切除术后一个月，向一组Ovx大鼠注射28.55 mg的葡萄糖酸庚二酸钙（Ca）/ kg b.w.，而另两组则单独用赋形剂进行长期治疗（Ovx和假手术对照）。手术两个月后，将动物处死。在甲状腺C细胞中，降钙素（CT）通过过氧化物酶-抗过氧化物酶方法定位。体视学用于评估C细胞体积，细胞核和相对体积密度的形态变化。计算每单位面积的C单元的数量。通过放射免疫测定法测定血清CT含量。慢性Ca处理后，与假手术对照组动物的C细胞相比，C细胞的细胞质更暗，但比Ovx大鼠的相应细胞更脱粒。与相应的对照组相比，它们的体积显着减少了14％（p <0.05），而数量却增加了47％（p <0.05）。与假手术对照组相比，血清CT浓度降低了27％（n.s.）。与未治疗的Ovx动物相比，钙治疗Ovx大鼠导致血清CT浓度增加32％。这些结果表明，Ovx雌性大鼠的慢性Ca治疗积极影响了甲状腺C细胞的CT释放，而形态学参数没有任何显着变化。

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