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首页> 外文期刊>Life sciences >Protective effects of glutathione and cysteine on the methylmercury-induced striatal dopamine release in vivo
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Protective effects of glutathione and cysteine on the methylmercury-induced striatal dopamine release in vivo

机译：谷胱甘肽和半胱氨酸对甲基汞诱导的纹状体多巴胺体内释放的保护作用

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摘要

The possible protective effects of glutathione (GSH), cysteine (CYS) and methionine (MET) on the Methylmercury (MeHg)-induced dopamine (DA) release from rat striatum were investigated using in vivo microdialysis coupled to HPLC with electrochemical detection. Intrastriatal infusion of MeHg 400 PM increased extracellular DA levels to 1941 +/- 199% in terms of basal levels. Infusion of MeHg 400 mu M in GSH 400 mu M pretreated animals, only increased striatal DA levels to 465 +/- 104% in terms of basal levels, this increase being 76% lower than induced by MeHg alone. Conversely, the infusion of MeHg 400 mu M after infusion of GSH 400 mu M increased DA levels to 1019 +/- 96% in terms of basal levels, this increase being 47.5% lower than that observed in MeHg non-pretreated animals. The infusion of MeHg 400 mu M in CYS 400 mu M -pretreated animals, increased striatal DA levels to 740 +/- 149%, in terms of basal levels, this increase being 62% lower than that induced by MeHg in non-pretreated animals. The infusion of MeHg 400 mu M in MET 400 mu M pretreated animals increased striatal DA levels to 2011 +/- 230% in terms of basal, an increase that was not significantly different from that produced by MeHg 400 mu M alone. In summary, the administration Of Compounds containing free -SH groups prevented the MeHg-induced DA release from rat striatum, probably due to the binding of MeHg to -SH groups. This would result in a lower metal availability to interact with SH membrane proteins groups, which would decrease MeHg ability to interact with DA transporter. (c) 2005 Elsevier Inc. All fights reserved.
机译：使用体内微量渗析结合HPLC和电化学检测研究了谷胱甘肽（GSH），半胱氨酸（CYS）和蛋氨酸（MET）对甲基汞（MeHg）诱导的大鼠纹状体释放多巴胺（DA）释放的可能保护作用。 MeHg 400 PM的纹状体内输注使基础水平方面的细胞外DA水平增加至1941 +/- 199％。在GSH 400μM预处理动物中输注400μMMeHg，仅基础水平将纹状体DA水平提高至465 +/- 104％，比仅由MeHg诱导的降低了76％。相反，以基础水平计，输注400μMGSH后输注400μMMeHg使DA水平增加至基础水平的1019 +/- 96％，比未进行过MeHg预处理的动物低47.5％。在经CYS 400μM预处理的动物中注入400μMMeHg，以基础水平计，纹状体DA水平增加至740 +/- 149％，比未预处理的动物中MeHg诱导的水平低62％ 。在MET 400μM预处理的动物中注入MeHg 400μM，以纹状体计，纹状体DA水平增加至2011 +/- 230％，这一增加与单独使用MeHg 400μM产生的增加无明显差异。总之，含有游离-SH基团的化合物的给药阻止了MeHg诱导的DA从大鼠纹状体释放，这可能是由于MeHg与-SH基团的结合。这将导致与SH膜蛋白基团相互作用的金属利用率降低，这将降低MeHg与DA转运蛋白相互作用的能力。 （c）2005 Elsevier Inc.保留所有战斗权。

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