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首页> 外文期刊>Life sciences >Propolis protects human spermatozoa from DNA damage caused by benzo(a)pyrene and exogenous reactive oxygen species.
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Propolis protects human spermatozoa from DNA damage caused by benzo(a)pyrene and exogenous reactive oxygen species.

机译：蜂胶可保护人类精子免受苯并（a）re和外源性活性氧造成的DNA损伤。

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摘要

Many environmental, physiological and genetic factors have been implicated in defective sperm function, the most common cause of infertility. In addition, sperm preparation techniques such as centrifugation, used prior to in vitro fertilization, are associated with the generation of reactive oxygen species (ROS) and an increase in the level of DNA damage. Factors that can offer spermatozoa protection are, therefore, of great importance. This study was designed to examine in vitro the effect of a Chilean propolis ethanolic extract on human spermatozoa treated with benzo[a]pyrene and exogenous reactive oxygen species. Our experimental evidence demonstrated that the natural drug under investigation is able to protect genomic DNA by damage induced by benzo[a]pyrene, hydrogen peroxide (H2O2) and hydrogen peroxide in combination with adenosine 5'-diphosphate (ADP) and ferrous sulfate (FeSO4), determining a significant reduction of the intracellular oxidants. An increase in membrane damage, measured by monitoring the formation of thiobarbituric acid-reactive substances (TBARS) and lactic dehydrogenase (LDH) release, was observed only in sperm treated with H2O2, ADP and FeSO4. The propolis extract was shown to possess the capacity to protect sperm membrane from the deleterious action of oxidative attack, reducing TBARS formation and LDH release. In summary, our results evidence that the protective effect exhibited by this natural compound in human spermatozoa is correlated, at least in part, to the antioxidant capacity of its active components, and suggest that propolis may have a role in protection against male infertility.
机译：许多环境，生理和遗传因素都与精子功能缺陷有关，这是不育的最常见原因。另外，在体外受精之前使用的诸如离心等精子制备技术与活性氧（ROS）的产生和DNA损伤水平的提高有关。因此，可以提供保护精子的因素非常重要。本研究旨在体外研究智利蜂胶乙醇提取物对苯并[a] re和外源性活性氧处理过的人精子的影响。我们的实验证据表明，所研究的天然药物能够通过苯并[a] ,、过氧化氢（H2O2）和过氧化氢与5'-二磷酸腺苷（ADP）和硫酸亚铁（FeSO4）组合引起的损伤来保护基因组DNA。 ），确定细胞内氧化剂的显着减少。通过监测硫代巴比妥酸反应性物质（TBARS）和乳酸脱氢酶（LDH）释放的形成来测量膜损伤的增加，仅在用H2O2，ADP和FeSO4处理的精子中观察到。蜂胶提取物被证明具有保护精子膜免受氧化攻击的有害作用，减少TBARS形成和LDH释放的能力。总之，我们的结果证明了这种天然化合物在人类精子中表现出的保护作用至少部分与其活性成分的抗氧化能力有关，并且表明蜂胶可能在抵抗男性不育症中起作用。

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