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首页> 外文期刊>Cell and Tissue Research >Cloning of corticotropin-releasing hormone (CRH) precursor cDNA and immunohistochemical detection of CRH peptide in the brain of the Japanese eel, paying special attention to gonadotropin-releasing hormone
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Cloning of corticotropin-releasing hormone (CRH) precursor cDNA and immunohistochemical detection of CRH peptide in the brain of the Japanese eel, paying special attention to gonadotropin-releasing hormone

机译：鳗el大脑中促肾上腺皮质激素释放激素（CRH）前体cDNA的克隆和CRH肽的免疫组织化学检测，尤其要注意促性腺激素释放激素

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摘要

The stress-related corticotropin-releasing hormone (CRH) was first identified by isolation of its cDNA from the brain of the Japanese eel Anguilla japonica. CRH cDNA encodes a signal peptide, a cryptic peptide and CRH (41 amino acids). The sequence homology to mammalian CRH is high. Next, the distribution of CRH-immunoreactive (ir) cell bodies and fibers in the brain and pituitary were examined by immunohistochemistry. CRH-ir cell bodies were detected in several brain regions, e.g., nucleus preopticus pars magnocellularis, nucleus preopticus pars gigantocellularis and formatio reticularis superius. In the brain, CRH-ir fibers were distributed not only in the hypothalamus but also in various regions. Some CRH-ir fibers projected to adrenocorticotropic hormone (ACTH) cells in the rostral pars distalis of the pituitary and also the α-melanocyte-stimulating hormone (α-MSH) cells in the pars intermedia of the pituitary. Finally, the neuroanatomical relationship between the CRH neurons and gonadotropin-releasing hormone (GnRH) neurons was examined by dual-label immunohistochemistry. CRH-ir fibers were found to be in close contact with GnRH-ir cell bodies in the hypothalamus and in the midbrain tegmentum and GnRH-ir fibers were in close contact with CRH-ir cell bodies in the nucleus preopticus pars magnocellularis. These results suggest that CRH has some physiological functions other than the stimulation of ACTH and α-MSH secretion and that reciprocal connections may exist between the CRH neurons and GnRH neurons in the brain of the Japanese eel.
机译：压力相关的促肾上腺皮质激素释放激素（CRH）首先是通过从日本鳗An的大脑中分离cDNA来鉴定的。 CRH cDNA编码信号肽，隐性肽和CRH（41个氨基酸）。与哺乳动物CRH的序列同源性很高。接下来，通过免疫组织化学检查了CRH免疫反应性（ir）细胞体和纤维在脑和垂体中的分布。在几个脑区域中检测到CRH-ir细胞体，例如，最适细胞核为大细胞核，最适细胞核为大细胞核和网状format。在大脑中，CRH-ir纤维不仅分布在下丘脑，而且分布在各个区域。一些CRH-ir纤维投射到垂体鼻旁部的促肾上腺皮质激素（ACTH）细胞，以及垂体中间pars中的α-黑素细胞刺激激素（α-MSH）细胞。最后，通过双标记免疫组织化学检查了CRH神经元和促性腺激素释放激素（GnRH）神经元之间的神经解剖关系。发现CRH-ir纤维与下丘脑和中脑被膜中的GnRH-ir细胞体紧密接触，而GnRH-ir纤维与白细胞前核中的CRH-ir细胞体紧密接触。这些结果表明CRH除了刺激ACTH和α-MSH分泌外还具有某些生理功能，并且在日本鳗鱼的大脑中CRH神经元和GnRH神经元之间可能存在相互联系。

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