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首页> 外文期刊>Life sciences >Involvement of monoamine oxidase inhibition in neuroprotective and neurorestorative effects of Ginkgo biloba extract against MPTP-induced nigrostriatal dopaminergic toxicity in C57 mice.
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Involvement of monoamine oxidase inhibition in neuroprotective and neurorestorative effects of Ginkgo biloba extract against MPTP-induced nigrostriatal dopaminergic toxicity in C57 mice.

机译：单胺氧化酶抑制作用涉及银杏叶提取物对MP57诱导的C57小鼠黑质纹状体多巴胺能毒性的神经保护和神经修复作用。

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摘要

The present study investigated the neuroprotective and neurorestorative effects of Ginkgo biloba extract (EGb 761) and its two components ginkgolides A (BN52020) and B (BN52021) in mice. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) (30 mg/kg/d i.p. for six days) significantly reduced striatal dopamine (DA) levels in C57 mice measured by high performance liquid chromatography with electrochemical detection (HPLC-EC). When C57 mice were pretreated with EGb 761 (20, 50, 100 mg/kg/d i.p.) for 7 days and then treated with the same extract 30 min before MPTP injection for 6 days, the neurotoxic effect of MPTP was antagonized in a dose-dependent fashion. Similar treatment with ginkgolides A and B (5, 10, 50 mg/kg/d i.p.) showed no protective effect. When C57 mice were treated with EGb 761 (50 mg/kg/d i.p.) after MPTP-lesion, the recovery of striatal dopamine (DA) levels was accelerated. However, similar treatment with ginkgolides A or B (10 mg/kg/d i.p.) did not show any effect. EGb 761, but not ginkgolides A and B, nonselectively inhibited mouse brain MAO activity in vitro (IC50 = 36.45 +/- 1.56 microg/ml) tested by an improved fluorimetric assay. The results demonstrate that EGb 761 administered before or after MPTP treatment effectively protects against MPTP-induced nigrostriatal dopaminergic neurotoxicity and that the inhibitory effect of EGb 761 on brain MAO may be involved in its neuroprotective effect.
机译：本研究调查了银杏叶提取物（EGb 761）及其两个成分银杏内酯A（BN52020）和B（BN52021）在小鼠中的神经保护和神经修复作用。 1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）（30 mg / kg / d ip连续六天）通过高效液相色谱法测定C57小鼠的纹状体多巴胺（DA）水平电化学检测（HPLC-EC）。当C57小鼠用EGb 761（20、50、100 mg / kg / d ip ip）预处理7天，然后在MPTP注射6天前30分钟用相同的提取物处理，其剂量拮抗了MPTP的神经毒性作用依赖的时尚。用银杏内酯A和B（5、10、50 mg / kg / d i.p.）进行的类似处理没有保护作用。当MPTP损伤后用EGb 761（50 mg / kg / d i.p.）治疗C57小鼠时，纹状体多巴胺（DA）水平的恢复得以加速。但是，用银杏内酯A或B（10 mg / kg / d i.p.）进行的类似治疗未见任何效果。通过改进的荧光测定法测试，EGB 761而非银杏内酯A和B没有非选择性地抑制小鼠体外MAO活性（IC50 = 36.45 +/- 1.56 microg / ml）。结果表明，在MPTP治疗之前或之后施用的EGb 761可有效预防MPTP诱导的黑纹状体多巴胺能神经毒性，并且EGb 761对脑MAO的抑制作用可能与其神经保护作用有关。

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