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首页> 外文期刊>Life sciences >Impact of treadmill exercise on early apoptotic cells in mouse thymus and spleen.
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Impact of treadmill exercise on early apoptotic cells in mouse thymus and spleen.

机译：跑步机运动对小鼠胸腺和脾脏早期凋亡细胞的影响。

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Lymphocyte apoptosis occurs in response to stressors such as thermal injury, trauma, sepsis, and surgery. This study evaluated the effect of a single bout of physical exercise stress on the induction of apoptosis in murine thymocytes and splenic lymphocytes. Female C57BL/6 mice, treadmill exercised at a submaximal intensity (35 m/min, 6% grade) for 90 min or serving as controls (walking on treadmill at 12 m/min, 6% grade, 5 min), were sacrificed 5 min or 120 min after completion of exercise. The percent of apoptotic, necrotic, and viable thymocytes and splenocytes were determined by flow cytometry using annexin V FITC and propidium iodide. There was a significantly higher percent of viable splenocytes in the mice sampled 120 min after cessation of exercise than treadmill control animals (p<0.05). In the thymus, there was a significantly lower percent of apoptotic (p<0.5) and a significantly higher percent of viable (p<0.05) cells in exercised mice sampled at 120 min after exercise relative to controls. Absolute numbers of thymocytes and splenocytes did not differ by exercise treatment condition. Plasma corticosterone levels were elevated immediately after exercise and were negatively correlated with the percent of viable lymphocytes in the spleen. During the time frame sampled, submaximal exercise is associated with a lower % of thymocytes expressing early markers of apoptosis, despite elevated plasma corticosterone levels. Retention of self-reactive, viable thymocytes which would normally be deleted or selective trafficking of apoptotic thymocytes out of the thymus may be involved in the exercise effect. Additional studies are necessary to identify the mechanisms for this shift in proportions of apoptotic and viable cells in lymphoid compartments with exercise.
机译：淋巴细胞凋亡是对压力的响应，例如热损伤，创伤，败血症和手术。这项研究评估了单次体育锻炼对小鼠胸腺细胞和脾淋巴细胞凋亡的诱导作用。处死雌性C57BL / 6小鼠，在亚最大强度（35 m / min，6％坡度）上跑步90分钟或作为对照（以12 m / min，6％坡度，5分钟在跑步机上行走）5运动结束后的120分钟或120分钟。使用膜联蛋白V FITC和碘化丙啶通过流式细胞术确定凋亡，坏死和存活的胸腺细胞和脾细胞的百分比。在运动停止后120分钟采样的小鼠中，脾脏活细胞百分比明显高于跑步机对照组动物（p <0.05）。相对于对照，在运动后120分钟采样的运动小鼠中，胸腺中凋亡细胞的百分比（p <0.5）显着降低，而存活细胞的百分比（p <0.05）则显着更高。胸腺细胞和脾细胞的绝对数量在运动治疗条件下没有差异。运动后血浆皮质酮水平立即升高，并且与脾脏中活淋巴细胞的百分比呈负相关。在采样的时间范围内，尽管血浆皮质酮水平升高，次最大运动与表达细胞凋亡早期标记物的胸腺细胞百分比降低有关。保留自我反应的，有活力的胸腺细胞（通常会被删除）或凋亡胸腺细胞有选择地从胸腺中运出可能与锻炼效果有关。有必要进行其他研究来确定运动引起的淋巴区室中凋亡和存活细胞比例发生这种变化的机制。

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