• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Morphine treatment affects the regulation of high mobility group I-type chromosomal phosphoproteins in C6 glioma cells.
【24h】

Morphine treatment affects the regulation of high mobility group I-type chromosomal phosphoproteins in C6 glioma cells.

机译：吗啡治疗会影响C6胶质瘤细胞中高迁移性I型染色体磷蛋白的调节。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

High mobility group (HMG) I-type chromosomal phosphoproteins HMG I/Y and HMG I-C were investigated following morphine treatment of C6BU-1 glioma. Cells were labelled with [32P]-orthophosphoric acid. Electrophoretic profiles and autoradiograms of the control cells revealed the presence of HMG I and HMG I-C proteins. HMG Y was not detected. Northern blot analysis showed a single HMG I/Y transcript. Treatment with morphine lowered the [32P]-incorporation in HMG I and HMGI-C proteins and the level of the HMG I/Y transcript. However, it did not change the protein ratios on the Coomassie stained gels. These results suggest that morphine may trigger independent reaction pathways affecting either transcription regulation and/or postsynthetic phosphorylation of the preexisting HMG I-type proteins. In addition, opposing changes in the postsynthetic phosphorylation of HMG 14 and histones H1AB were also noticed.
机译：吗啡治疗C6BU-1胶质瘤后，研究了高迁移率族（HMG）I型染色体磷蛋白HMG I / Y和HMG I-C。细胞用[32P]-正磷酸标记。对照细胞的电泳图谱和放射自显影图显示了HMG I和HMG I-C蛋白的存在。未检测到HMGY。 Northern印迹分析显示单个HMG I / Y转录物。吗啡处理降低了HMG I和HMGI-C蛋白中的[32P]掺入以及HMG I / Y转录本的水平。但是，它没有改变考马斯染色凝胶上的蛋白质比例。这些结果表明吗啡可能会触发独立的反应途径，从而影响先前存在的HMG I型蛋白的转录调控和/或合成后磷酸化。另外，还注意到HMG 14和组蛋白H1AB的合成后磷酸化中的相反变化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号