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首页> 外文期刊>Cell and Tissue Research >Expression of insulin-like factor 3 hormone-receptor system in the reproductive organs of male goats
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Expression of insulin-like factor 3 hormone-receptor system in the reproductive organs of male goats

机译：胰岛素样因子3激素受体在雄性山羊生殖器官中的表达

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摘要

Relaxin-like factor (RLF), generally known as insulin-like factor 3 (INSL3), is essential for testis descent during fetal development. However, its role in adult males is not fully understood. We investigate the function of INSL3 in male Saanen goats by identifying cell types expressing its receptor, relaxin/insulin-like family peptide receptor (RXFP)2 and by characterizing the developmental expression pattern of INSL3 and RXFP2 and the binding of INSL3 to target cells in the male reproductive system. A highly specific RXFP2 antibody that co-localizes with an anti-FLAG antibody in HEK-293 cells recognizes RXFP2-transcript-expressing cells in the testis. INSL3 and RXFP2 mRNA expression is upregulated in the testis, starting from puberty. INSL3 mRNA and protein expression has been detected in Leydig cells, whereas RXFP2 mRNA and protein localize to Leydig cells, to meiotic and post-meiotic germ cells and to the epithelium and smooth muscle of the cauda epididymis and vas deferens. INSL3 binds to all of these tissues and cell types, with the exception of Leydig cells, in a hormone-specific and saturable manner. These results provide evidence for a functional intra- and extra-testicular INSL3 ligand-receptor system in adult male goats.
机译：松弛素样因子（RLF），通常称为胰岛素样因子3（INSL3），对于胎儿发育期间的睾丸下降至关重要。但是，其在成年男性中的作用尚未完全了解。我们通过鉴定表达其受体，松弛素/胰岛素样家族肽受体（RXFP）2的细胞类型，并表征INSL3和RXFP2的发育表达模式以及INSL3与靶细胞的结合，来研究INSL3在雄性Saanen山羊中的功能。男性生殖系统。在HEK-293细胞中与抗FLAG抗体共定位的高度特异性RXFP2抗体可识别睾丸中表达RXFP2的细胞。从青春期开始，INSL3和RXFP2 mRNA表达在睾丸中被上调。已在Leydig细胞中检测到INSL3 mRNA和蛋白表达，而RXFP2 mRNA和蛋白定位于Leydig细胞，减数分裂和减数分裂后的生殖细胞以及附睾和输精管的上皮和平滑肌。除Leydig细胞外，INSL3以激素特异性且可饱和的方式与所有这些组织和细胞类型结合。这些结果为成年雄性山羊的功能性睾丸内和睾丸外INSL3配体-受体系统提供了证据。

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