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首页> 外文期刊>Life sciences >Monitoring the correction of glycogen storage disease type 1a in a mouse model using [F-18]FDG and a dedicated animal scanner
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Monitoring the correction of glycogen storage disease type 1a in a mouse model using [F-18]FDG and a dedicated animal scanner

机译：使用[F-18] FDG和专用动物扫描仪监测小鼠模型中糖原贮积病1a型的校正

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Monitoring gene therapy of glycogen storage disease type I a in a mouse model was achieved using [F-18]FDG and a dedicated animal scanner. The G6Pase knockout (KO) mice were compared to the same mice after infusion with a recombinant adenovirus containing the murine G6Pase gene (Ad-mG6Pase). Serial images of the same mouse before and after therapy were obtained and compared with wild-type (WT) mice of the same strain to determine the uptake and retention of [F-18]FDG in the liver. Image data were acquired from heart, blood pool and liver for twenty minutes after injection of [F-18]FDG. The retention of [F-18]FDG was lower for the WT mice compared to the KO mice. The mice treated with adenovirus-mediated gene therapy had retention similar to that found in age-matched WT mice. These studies show that FDG can be used to monitor the G6Pase concentration in liver of WT mice as compared to G6Pase KO mice. In these mice, gene therapy returned the liver function to that found in age matched WT controls as measured by the FDG kinetics in the liver compared to that found in age matched wild type controls. Published by Elsevier Science Inc. [References: 32]
机译：使用[F-18] FDG和专用的动物扫描仪，可以在小鼠模型中监测I型糖原贮积病的基因治疗。将G6Pase基因敲除（KO）小鼠与含有鼠G6Pase基因（Ad-mG6Pase）的重组腺病毒输注后的小鼠进行了比较。获得了治疗前后相同小鼠的系列图像，并将其与相同品系的野生型（WT）小鼠进行比较，以确定[F-18] FDG在肝脏中的摄取和保留。注射[F-18] FDG后二十分钟从心脏，血池和肝脏中获取图像数据。与KO小鼠相比，WT小鼠的[F-18] FDG保留较低。用腺病毒介导的基因疗法治疗的小鼠具有与年龄匹配的野生型小鼠相似的保留能力。这些研究表明，与G6Pase KO小鼠相比，FDG可用于监测WT小鼠肝脏中G6Pase的浓度。在这些小鼠中，基因治疗使肝脏功能恢复到与年龄相匹配的野生型对照中所发现的功能相同，这是通过肝脏中的FDG动力学与年龄相匹配的野生型对照中所发现的相比较而得出的。由Elsevier Science Inc.发布[参考：32]

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