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首页> 外文期刊>Life sciences >Anti-proliferative and differentiation-inducing activities of the green tea catechin epigallocatechin-3-gallate (EGCG) on the human eosinophilic leukemia EoL-1 cell line.
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Anti-proliferative and differentiation-inducing activities of the green tea catechin epigallocatechin-3-gallate (EGCG) on the human eosinophilic leukemia EoL-1 cell line.

机译：绿茶儿茶素表没食子儿茶素-3-没食子酸酯（EGCG）对人嗜酸性粒细胞EoL-1细胞系的抗增殖和分化诱导活性。

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摘要

A novel approach for the treatment of leukemia is the differentiation therapy in which immature leukemia cells are induced to attain a mature phenotype when exposed to differentiation inducers, either alone or in combinations with other chemotherapeutic or chemopreventive drugs. Over the past decade, numerous studies indicated that green tea catechins (GTC) could suppress the growth and induce apoptosis on a number of human cancer cell lines. However, the differentiation-inducing activity of GTC on human tumors remains poorly understood. In the present study, the effect of the major GTC epigallocatechin-3-gallate (EGCG) on the proliferation and differentiation of a human eosinophilc leukemic cell line, EoL-1, was examined. Our results showed that EGCG suppressed the proliferation of the EoL-1 cells in a dose-dependent manner, with an estimated IC(50) value of 31.5 microM. On the other hand, EGCG at a concentration of 40 microM could trigger the EoL-1 cells to undergo morphological differentiation into mature eosinophil-like cells. Using RT-PCR and flow cytometry, it was found that EGCG upregulated the gene and protein expression of two eosinophil-specific granule proteins, the major basic protein (MBP) and eosinophil peroxidase (EPO), in EoL-1 cells. Taken together, our findings suggest that EGCG can exhibit anti-leukemic activity on a human eosinophilic cell line EoL-1 by suppressing the proliferation and by inducing the differentiation of the leukemia cells.
机译：一种治疗白血病的新方法是分化疗法，其中当单独暴露于分化诱导剂或与其他化学治疗或化学预防药物联合使用时，诱导未成熟的白血病细胞达到成熟的表型。在过去的十年中，大量研究表明，绿茶儿茶素（GTC）可以抑制许多人类癌细胞系的生长并诱导其凋亡。然而，人们对GTC对人肿瘤的分化诱导活性知之甚少。在本研究中，检查了主要的GTC表没食子儿茶素-3-没食子酸酯（EGCG）对人嗜酸性粒细胞白血病细胞系EoL-1增殖和分化的影响。我们的结果表明，EGCG以剂量依赖性方式抑制EoL-1细胞的增殖，估计IC（50）值为31.5 microM。另一方面，浓度为40 microM的EGCG可能触发EoL-1细胞进行形态学分化为成熟的嗜酸性粒细胞样细胞。使用RT-PCR和流式细胞仪，发现EGCG在EoL-1细胞中上调了两种嗜酸性粒细胞特异性颗粒蛋白（主要的碱性蛋白（MBP）和嗜酸性粒细胞过氧化物酶（EPO））的基因和蛋白表达。综上所述，我们的发现表明，EGCG可以通过抑制增殖和诱导白血病细胞的分化而对人嗜酸性细胞系EoL-1表现出抗白血病活性。

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