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Immunogenicity of superoxide radical modified-DNA: studies on induced antibodies and SLE anti-DNA autoantibodies

机译：超氧自由基修饰的DNA的免疫原性：诱导抗体和SLE抗DNA自身抗体的研究

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摘要

Superoxide anion radical (SAR) is formed in almost all aerobic cells and it is the most abundant species generated by several enzymatic and non-enzymatic pathways in mammalian tissues, leading to unfavorable alteration of biomolecules including DNA. The SAR-modified macromolecules have been implicated in several disease states including disorders of inflammation. The SAR-induced damage to DNA showed hyperchromicity, single strand breaks, decrease in melting temperature, and modification of bases. Superoxide modified-DNA in rabbits elicited high titer antibodies and showed diverse antigens binding characteristics. The induced antibodies recognized native DNA and other nucleic acid polymers. Anti-DNA IgG from SLE sera, purified on Protein-A-Sepharose matrix, exhibited increased recognition of superoxide anion radical modified-DNA than native DNA in competitive immunoassay. The visual formation of immune complex between induced antibodies and native DNA, and between SLE anti-DNA IgG and superoxide modified-DNA, is a clear indication of property sharing between SLE autoantibodies and experimentally induced antibodies against superoxide modified-DNA. (C) 2004 Elsevier Inc. All rights reserved.
机译：超氧阴离子自由基（SAR）几乎在所有需氧细胞中形成，它是哺乳动物组织中几种酶促和非酶促途径产生的最丰富的物种，导致包括DNA在内的生物分子发生不利变化。 SAR修饰的大分子与多种疾病有关，包括炎症。 SAR诱导的DNA损伤表现为高色度，单链断裂，熔解温度降低和碱基修饰。兔子体内的超氧化物修饰的DNA引发了高滴度的抗体，并显示出多种抗原结合特性。诱导的抗体识别天然DNA和其他核酸聚合物。在竞争性免疫测定中，在蛋白质-A-琼脂糖基质上纯化的SLE血清抗DNA IgG表现出比天然DNA更高的对超氧阴离子自由基修饰的DNA的识别。诱导抗体和天然DNA之间以及SLE抗DNA IgG和超氧化物修饰的DNA之间免疫复合物的视觉形成，清楚地表明了SLE自身抗体与实验诱导的抗超氧化物修饰的DNA抗体之间的属性共享。 （C）2004 Elsevier Inc.保留所有权利。

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