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Inhibition of xanthine oxidase by phytic acid and its antioxidative action

机译：植酸对黄嘌呤氧化酶的抑制作用及其抗氧化作用

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We examined if phytic acid inhibits the enzymatic superoxide. source xanthine oxidase (XO). Half inhibition of XO by phytic acid (IC50) was about 30 mM in the formation of uric acid from xanthine, but generation of the superoxide was greatly affected by phytic acid; the IC50 was about 6 mM, indicating that the superoxide generating domain of XO is more sensitive to phytic acid. The XO activity in intestinal homogenate was also inhibited by phytic acid. However, it was not observed with intestinal homogenate that superoxide generation was more sensitive to phytic acid compared with the formation of uric acid as observed with XO from butter milk. XO-induced superoxide-dependent lipid peroxidation was inhibited by phytic acid, but not by myo-inositol. Reduction of ADP-Fe3+ caused by XO was inhibited by superoxide dismutase, but not phytic acid. The results suggest that phytic acid interferes with the formation of ADP-iron-oxygen complexes that initiate lipid peroxidation. Both phytic acid and myo-inositol inhibited XO-induced superoxide-dependent DNA damage. Mannitol inhibited the DNA strand break. Myo-inositol may act as a hydroxyl radical scavenger. The antioxidative action of phytic acid may be due to not only inhibiting XO, but also preventing formation of ADP-iron-oxygen complexes. (C) 2003 Elsevier Inc. All rights reserved. [References: 32]
机译：我们检查了植酸是否抑制了酶促超氧化物。来源黄嘌呤氧化酶（XO）。从黄嘌呤形成尿酸时，植酸（IC50）对XO的抑制作用约为30 mM，但植酸极大地影响了超氧化物的生成; IC50约为6 mM，表明XO的超氧化物生成域对植酸更敏感。植酸还可抑制肠匀浆中的XO活性。但是，与从牛乳中XO观察到的尿酸形成相比，没有观察到与肠匀浆相比超氧化物生成对植酸更敏感。 XO诱导的超氧化物依赖性脂质过氧化被植酸抑制，但不受肌醇抑制。 XO引起的ADP-Fe3 +的还原被超氧化物歧化酶抑制，而植酸则没有。结果表明植酸会干扰ADP-铁-氧复合物的形成，从而引发脂质过氧化。植酸和肌醇均抑制XO诱导的超氧化物依赖性DNA损伤。甘露醇抑制DNA链断裂。肌醇可以充当羟基自由基清除剂。植酸的抗氧化作用可能不仅是由于抑制XO，而且还阻止了ADP-铁-氧络合物的形成。 （C）2003 Elsevier Inc.保留所有权利。 [参考：32]

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