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首页> 外文期刊>Life sciences >Antifibrotic effect of the Chinese herbs, Astragalus mongholicus and Angelica sinensis, in a rat model of chronic puromycin aminonucleoside nephrosis
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Antifibrotic effect of the Chinese herbs, Astragalus mongholicus and Angelica sinensis, in a rat model of chronic puromycin aminonucleoside nephrosis

机译：中草药黄芪和当归对慢性嘌呤霉素氨基核苷肾病大鼠模型的抗纤维化作用

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Nephrotic syndrome has long been treated in China with two herbs, Astragalus mongholicus and Angelica sinensis, which may have antifibrotic effects. Methods: Rats with chronic puromycin-induced nephrosis were treated with Astragalus and Angelica 3 mL/d (n = 7) or enalapril 10 mg/kg/d (n = 7). Normal control rats (n = 7) received saline rather than puromycin, and an untreated control group (n = 7) received puromycin but no treatment. After 12 weeks, stained sections of the glomerulus and tubulointerstitium were evaluated for injury. Immunohistochemistry staining measured extracellular matrix components, transforming growth factor-beta1 (TGFbeta1), osteopontin, ED-1-positive cells, and alpha-actin. TGF (a) over cap1 mRNA was assessed by in situ hybridization. Renin, ACE activity, angiotensin, and aldosterone were measured by radioimmunoassay or colorimetry. In the untreated rats, chronic renal injury progressed to marked fibrosis at 12 weeks. Astragalus and Angelica significantly reduced deterioration of renal function and histologic damage. Expressions of type III and IV collagen, fibronectin, and laminin also decreased significantly. This anti-fibrotic effect was similar to that of enalapril. The herbs had no effect on the renin-angiotensin system but did reduce the number of ED-1-positive, and alpha-actin positive cells and expression of osteopontin compared to untreated controls. The combination of Astragalus and Angelica retarded the progression of renal fibrosis and deterioration of renal function with comparable effects of enalapril These effects were not caused by blocking the intrarenal renin-angiotensin system, but associated with suppression of the overexpression of TGFbeta(1) and osteopontin, reduction of infiltrating macrophages, and less activation of renal intrinsic cells. (C) 2003 Elsevier Inc. All rights reserved. [References: 40]
机译：长期以来，中国一直用两种草药治疗肾病综合症：黄芪和当归，它们可能具有抗纤维化作用。方法：用黄芪和当归3 mL / d（n = 7）或依那普利10 mg / kg / d（n = 7）治疗慢性嘌呤霉素肾病大鼠。正常对照组（n = 7）接受生理盐水而不是嘌呤霉素，未治疗的对照组（n = 7）接受嘌呤霉素但未接受治疗。 12周后，评估肾小球和肾小管间质的染色切片的损伤。免疫组织化学染色测定了细胞外基质成分，转化生长因子-beta1（TGFbeta1），骨桥蛋白，ED-1阳性细胞和α-肌动蛋白。通过原位杂交评估了cap1 mRNA上的TGF（a）。肾素，ACE活性，血管紧张素和醛固酮通过放射免疫测定法或比色法测定。在未经治疗的大鼠中，慢性肾损伤在12周时发展为明显的纤维化。黄芪和当归显着减少了肾功能的恶化和组织学损害。 III型和IV型胶原，纤连蛋白和层粘连蛋白的表达也显着下降。这种抗纤维化作用类似于依那普利。与未处理的对照组相比，这些草药对肾素-血管紧张素系统没有影响，但确实减少了ED-1阳性和α-肌动蛋白阳性细胞的数量以及骨桥蛋白的表达。黄芪和当归的组合可抑制肾纤维化的进展和肾功能的恶化，同时具有依那普利的作用。这些作用不是由阻断肾内肾素-血管紧张素系统引起的，而是与抑制TGFbeta（1）和骨桥蛋白的过表达有关，减少浸润性巨噬细胞和较少激活肾脏内在细胞。 （C）2003 Elsevier Inc.保留所有权利。 [参考：40]

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