• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Herbal medicine Sho-saiko-to (TJ-9) increases expression matrix metalloproteinases (MMPs) with reduced expression of tissue inhibitor of metalloproteinases (TIMPs) in rat stellate cell
【24h】

Herbal medicine Sho-saiko-to (TJ-9) increases expression matrix metalloproteinases (MMPs) with reduced expression of tissue inhibitor of metalloproteinases (TIMPs) in rat stellate cell

机译：草药Sho-saiko-to（TJ-9）在大鼠星状细胞中增加了表达基质金属蛋白酶（MMP）的表达，并减少了组织金属蛋白酶（TIMPs）的表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have reported that Sho-saiko-to (TJ-9) prevents liver fibrosis in vivo. To gain further insights into the effect of TJ-9, the matrix metalloprotemases (MMPs)/tissue inhibitors of metalloproteinases (TIMPs) balance was examined. Hepatic stellate cells (HSCs) were isolated from male Wistar rats and cultured with TJ-9 (0-1000 mug/ml) on uncoated plastic dishes for 4 days. To elucidate the effects on the MMPs/TIMPs balance by TJ-9, quantitative analysis of type IV collagen-degrading activity, gelatin zymography and reverse zymography were carried out. Northern blot analysis was performed to determine the expression of MMP-2, 13 and TIMP-1 mRNAs. TJ-9 treatment resulted in dose-dependent upregulation of MMP-2, 13 mRNA and downregulation of TIMP-1 mRNA up to 500 mug/ml. Gelatin zymography, reverse zymography and quantitative analysis of type IV collagen-degrading activity confirmed that TJ-9 increased MMP-2 activity and prevented TIMP-1, 2 activities in a dose-dependent manner. SB203580 diminished the reduction of mRNA as well as the activity of TIMP-I by TJ-9 and induction of mRNA as well as the activity of MMP-2. These results show that TJ-9 increased MMP-2, 13 activity with reduced TIMP-1, 2 activities on HSCs possibly via P38 pathway. (C) 2004 Elsevier Inc. All rights reserved. [References: 44]
机译：我们已经报道说Sho-saiko-to（TJ-9）可以在体内预防肝纤维化。为了进一步了解TJ-9的作用，研究了基质金属蛋白酶（MMP）/金属蛋白酶组织抑制剂（TIMPs）平衡。从雄性Wistar大鼠中分离出肝星状细胞（HSC），并在未涂覆的塑料皿中与TJ-9（0-1000杯/毫升）一起培养4天。为了阐明TJ-9对MMP / TIMPs平衡的影响，对IV型胶原降解活性，明胶酶谱和反向酶谱进行了定量分析。进行Northern印迹分析以确定MMP-2、13和TIMP-1 mRNA的表达。 TJ-9处理导致MMP-2、13 mRNA的剂量依赖性上调和TIMP-1 mRNA的下调，直至500 mug / ml。明胶酶谱，反向酶谱和IV型胶原降解活性的定量分析证实，TJ-9以剂量依赖性方式增加了MMP-2活性并阻止了TIMP-1、2活性。 SB203580减少了TJ-9对mRNA的还原以及TIMP-1的活性以及对mRNA的诱导以及MMP-2的活性。这些结果表明，TJ-9可能通过P38途径增加了HSC的MMP-2、13活性和TIMP-1、2活性。 （C）2004 Elsevier Inc.保留所有权利。 [参考：44]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号