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首页> 外文期刊>Life sciences >Epigallocatechin gallate induces telomere fragmentation in HeLa and 293 but not in MRC-5 cells.
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Epigallocatechin gallate induces telomere fragmentation in HeLa and 293 but not in MRC-5 cells.

机译：表没食子儿茶素没食子酸酯在HeLa和293中诱导端粒断裂，但在MRC-5细胞中不诱导。

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摘要

Telomeres are the tandem repetitive sequence at the end of chromosomes and its integrity is crucial for cell vitality. We studied the effect of (-)-epigallocatechin-3-gallate (EGCG), one of the major tea polyphenols, on telomeres in HeLa, 293 cells and MRC-5 fibroblasts. At concentrations of above 50 microM, EGCG was found to causes telomere fragmentation in HeLa cells as a result of single-strand breaks in a dose-dependent manner. Treatment of EGCG also caused telomere fragmentation in 293 cells but had little or only marginal effect on MRC-5 fibroblasts. The telomere fragments detected by electrophoresis showed a unique size distribution that seems to suggest that the strand breaks were not produced randomly, but with preference at some specific sites. We speculate that the differential effect of EGCG in inducing telomere fragmentation in HeLa and 293 verse MRC-5 cells might be relevant to the apoptosis-inducing effect of EGCG on cancerous cells but not on normal cells.
机译：端粒是染色体末端的串联重复序列，其完整性对于细胞活力至关重要。我们研究了主要茶多酚之一（-）-epigallocatechin-3-gallate（EGCG）对HeLa，293细胞和MRC-5成纤维细胞端粒的影响。在高于50 microM的浓度下，发现EGCG由于单链断裂以剂量依赖的方式在HeLa细胞中引起端粒断裂。 EGCG的处理还导致293细胞端粒断裂，但对MRC-5成纤维细胞影响很小或仅有很小的影响。电泳检测到的端粒片段显示出独特的大小分布，这似乎表明链断裂不是随机产生的，而是在某些特定位点优先产生。我们推测，EGCG诱导HeLa和293节MRC-5细胞端粒断裂的差异作用可能与EGCG对癌细胞的凋亡诱导作用有关，而与正常细胞无关。

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