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首页> 外文期刊>Life sciences >THE EFFECT OF HUMAN RECOMBINANT ERYTHROPOIETIN ON THE GROWTH OF A HUMAN NEUROBLASTOMA CELL LINE
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THE EFFECT OF HUMAN RECOMBINANT ERYTHROPOIETIN ON THE GROWTH OF A HUMAN NEUROBLASTOMA CELL LINE

机译：重组人促红细胞生成素对人神经母细胞瘤细胞系生长的影响

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摘要

Erythropoietin is a growth factor. Cancer can be described as disturbance of the fine balance of positive and negative growth control mechanisms. The effect of human recombinant erythropoietin (EPO) was studied on the cell growth and differentiation of a human neuroblastoma cell line (h-NMB). Cell growth curves, trypan blue staining and thymidine uptake were used to assess cell proliferation and death. To assess cell differentiation, neutral endopeptidase (cell membrane enzyme marker), creatine kinase (cytosolic enzyme marker), dopamine uptake (dopamine transporter marker) and cell morphology were determined. Specific EPO receptor mRNA, by RT-PCR technique, was demonstrated. The incubation of erythropoietin with the tumor cell line resulted in inhibition of cell proliferation as evidenced in a diminished cell growth. EPO was shown to have induced a differentiation process as seen from the two different enzymatic markers, membranal and cytosolic, and from the cells dopamine uptake studies. However, the morphological changes did not document a full differentiation effect. EPO specific antibodies blocked the effects of EPO on cell proliferation and creatine kinase activity. In this study, EPO did not produce any sign of proliferation in the nervous tumor cell line used. [References: 27]
机译：促红细胞生成素是一种生长因子。癌症可被描述为对正向和负向生长控制机制精细平衡的干扰。研究了人类重组促红细胞生成素（EPO）对人类神经母细胞瘤细胞系（h-NMB）细胞生长和分化的影响。细胞生长曲线，锥虫蓝染色和胸腺嘧啶核苷摄取被用来评估细胞增殖和死亡。为了评估细胞分化，确定了中性内肽酶（细胞膜酶标志物），肌酸激酶（胞质酶标志物），多巴胺摄取（多巴胺转运蛋白标志物）和细胞形态。通过RT-PCR技术证实了特定的EPO受体mRNA。促红细胞生成素与肿瘤细胞系的温育导致细胞增殖的抑制，如细胞生长减少所证明的。从两种不同的酶标记物（膜和胞质）以及细胞多巴胺摄取研究中可以看出，EPO诱导了分化过程。但是，形态变化没有证明完全的分化作用。 EPO特异性抗体阻断了EPO对细胞增殖和肌酸激酶活性的影响。在这项研究中，EPO在使用的神经肿瘤细胞系中未产生任何增殖迹象。 [参考：27]

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