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首页> 外文期刊>Life sciences >Characterization of the human nicotinic acetylcholine receptor subunit alpha (alpha) 9 (CHRNA9) and alpha (alpha) 10 (CHRNA10) in lymphocytes.
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Characterization of the human nicotinic acetylcholine receptor subunit alpha (alpha) 9 (CHRNA9) and alpha (alpha) 10 (CHRNA10) in lymphocytes.

机译：淋巴细胞中人烟碱型乙酰胆碱受体亚基α（α）9（CHRNA9）和α（α）10（CHRNA10）的特征。

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摘要

Though the nicotinic acetylcholine receptor (nAChR) subunits alpha9 and alpha 10 have been thoroughly characterized within hair cells of the organ of Corti in the inner ear, prior studies have shown that they are also expressed in lymphocytes. In this report, we sought to more definitively characterize the nAChR subunits alpha9 and alpha10 within various populations of human lymphocytes. Using a combination of techniques, including RT-PCR, single-cell RT-PCR, Northern and western blot analysis, and immunofluorescence, expression of both alpha9 and alpha 10 was demonstrated in purified populations of T-cells (CD3+, CD4+, CD8+ and the Jurkat, MT2 and CEM T-cell lines) and B-cells (CD19+, CD80+ and EBV-immortalized B-cells). Single-lymphocyte recording techniques failed to identify an ionic current in response to applied acetylcholine in either T-cells or B-cells. These results clearly demonstrate the presence of these nicotinic receptor subunits within several populations of human lymphocytes, implicating their role in the immune response. However, a lack of demonstrated response to applied acetylcholine using standard single-cell recording techniques suggests a physiology different than that seen in hair cells of the inner ear.
机译：尽管尼古丁乙酰胆碱受体（nAChR）亚基alpha9和alpha 10在内耳Corti器官的毛细胞中已得到充分表征，但先前的研究表明它们也在淋巴细胞中表达。在本报告中，我们试图更明确地表征人类淋巴细胞各种种群中的nAChR亚基alpha9和alpha10。使用多种技术的组合，包括RT-PCR，单细胞RT-PCR，Northern和Western blot分析以及免疫荧光，在纯化的T细胞群体（CD3 +，CD4 +，CD8 +和Jurkat，MT2和CEM T细胞系）和B细胞（CD19 +，CD80 +和EBV永生化B细胞）。单淋巴细胞记录技术无法响应T细胞或B细胞中施加的乙酰胆碱来识别离子电流。这些结果清楚地证明了这些烟碱样受体亚基存在于人类淋巴细胞的几个群体中，暗示了它们在免疫应答中的作用。然而，缺乏使用标准的单细胞记录技术对所施加的乙酰胆碱的反应的证实，表明其生理学不同于内耳的毛细胞中观察到的生理学。

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