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首页> 外文期刊>Life sciences >SYNERGISTIC ANTITUMOR ACTIVITY OF VITAMINS C AND K-3 ON HUMAN UROLOGIC TUMOR CELL LINES
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SYNERGISTIC ANTITUMOR ACTIVITY OF VITAMINS C AND K-3 ON HUMAN UROLOGIC TUMOR CELL LINES

机译：维生素C和K-3在人类泌尿肿瘤细胞系上的协同抗肿瘤活性

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摘要

A micro-tetrazolium assay was employed to evaluate vitamin C (VC), vitamin K-3 (VK3) and vitamin C/vitamin K-3 combinations (VC/VK3) for their antitumor activity against eight human urologic tumor cell lines. While the individual vitamins exhibited antitumor activity at high concentrations, co-administration of the vitamin in a VC : VK3 ratio of 100 : 1 potentiated antitumor activity 4- to 61-fold even when exposure times were as short as 1 hour. Administration of exogenous catalase destroyed the antitumor activity of the vitamins and suggested that catalase destroyed the antitumor activity of the vitamins and suggested that hydrogen peroxide and perhaps other reactive oxygen species were involved in the antitumor mechanism of these vitamins. Electron micrographs taken in a previous study demonstrated that vitamin treatment damaged mitochondria and may have impaired ATP synthesis. Analysis of cellular ATP and thiol levels as well as DNA and protein synthesis during the first five hours following a one hour VC/VK3 treatment, revealed: a transient increase in ATP production, a substantial decrease in DNA synthesis, an increase in protein synthesis and a decrease in thiol levels. These results suggested that redox cycling of the vitamin combination increased oxidative stress until it surpassed the reducing ability of the cellular thiols and cellular or genetic damage ensured. [References: 36]
机译：微型四唑鎓测定用于评估维生素C（VC），维生素K-3（VK3）和维生素C /维生素K-3组合（VC / VK3）对八种人类泌尿系统肿瘤细胞系的抗肿瘤活性。尽管各种维生素在高浓度下均表现出抗肿瘤活性，但即使在暴露时间短至1小时的情况下，以VC：VK3为100：1的比例共同施用维生素也能使抗肿瘤活性提高4至61倍。外源过氧化氢酶的施用破坏了维生素的抗肿瘤活性，并暗示过氧化氢酶破坏了维生素的抗肿瘤活性，并暗示过氧化氢和也许其他活性氧物种参与了这些维生素的抗肿瘤机理。先前研究中拍摄的电子显微照片表明，维生素处理破坏了线粒体，并可能破坏了ATP的合成。经过一小时的VC / VK3处理后的前五个小时，对细胞ATP和硫醇水平以及DNA和蛋白质合成的分析显示：ATP产量短暂增加，DNA合成显着下降，蛋白质合成和硫醇水平降低。这些结果表明，维生素组合物的氧化还原循环增加了氧化应激，直至其超过了细胞硫醇的还原能力并确保了细胞或遗传损伤。 [参考：36]

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