• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Bromelain improves decrease in defecation in postoperative rats: modulation of colonic gene expression of inducible nitric oxide synthase.
【24h】

Bromelain improves decrease in defecation in postoperative rats: modulation of colonic gene expression of inducible nitric oxide synthase.

机译：菠萝蛋白酶改善术后大鼠排便的减少：调节可诱导型一氧化氮合酶的结肠基因表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ileus continues to be a common consequence of abdominal surgery, causing significant patient discomfort and often leading to more serious problems. The therapy available is limited, hence, ileus remains an important clinical problem. Activation of inducible nitric oxide synthase (iNOS) directly modulates intestinal dysmotility after bowel manipulation and plays an essential role in initiating intestinal inflammation. Nuclear factor (NF)-kappaB is known to be a critical component of iNOS gene transcriptional activation in response to inflammatory stimuli. Bromelain is a crude extract from the pineapple stem, which is sold as a nutritional supplement to "promote digestive health" and as an anti-inflammatory medication in some developed countries. Here, we have found that oral administration of bromelain improves decrease in defecation in abdominal postoperative rats. Results showed that bromelain increased the wet weight, dry weight, water content and number of fecal pellets in laparotomized plus mechanically manipulated rats, suggesting improvement of postoperative ileus. Furthermore, bromelain treatment inhibited overexpressed iNOS mRNA and restored down-regulated inhibitor kappaBalpha mRNA in the colon of the postoperative rats. From the in vitro experiments, bromelain inhibits lipopolysaccharide (LPS)-induced nitrite overproduction in macrophage cell lines and LPS-induced NF-kappaB luciferase reporter gene expression in RAW264.7 macrophages transfected with NF-kappaB luciferase reporter gene. Thus, our findings suggest that bromelain improves decrease in defecation in postoperative rats, at least in part, by inhibiting colonic iNOS overexpression via NF-kappaB pathway. Our data indicates that bromelain may benefit patients with postoperative ileus.
机译：肠梗阻仍然是腹部手术的常见结果，导致患者明显不适，并经常导致更严重的问题。可用的疗法是有限的，因此，肠梗阻仍然是重要的临床问题。肠道操作后，诱导型一氧化氮合酶（iNOS）的激活直接调节肠道运动障碍，并在引发肠道炎症中起重要作用。已知核因子（NF）-kappaB是iNOS基因转录激活响应炎症刺激的关键组成部分。菠萝蛋白酶是菠萝干的粗提物，在某些发达国家被出售为营养补充剂，以“促进消化健康”和消炎药的形式出售。在这里，我们发现口服菠萝蛋白酶改善了腹部术后大鼠排便的减少。结果表明，菠萝蛋白酶增加了开腹手术和机械操作大鼠的湿重，干重，水含量和粪便颗粒数，表明术后肠梗阻有所改善。此外，菠萝蛋白酶治疗抑制了术后大鼠结肠中iNOS mRNA的过表达并恢复了抑制剂kappaBalpha mRNA的下调。从体外实验中，菠萝蛋白酶抑制了脂多糖（LPS）诱导的巨噬细胞细胞系中亚硝酸盐的过度生产，以及LPS诱导的被NF-κB荧光素酶报道基因转染的RAW264.7巨噬细胞中的NF-κB荧光素酶报道基因的表达。因此，我们的发现表明菠萝蛋白酶至少部分地通过抑制经由NF-κB途径的结肠iNOS过表达来改善术后大鼠排便的减少。我们的数据表明菠萝蛋白酶可以使术后肠梗阻患者受益。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号