• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >La autoantigen translocates to cytoplasm after cleavage during granzyme B-mediated cytotoxicity
【24h】

La autoantigen translocates to cytoplasm after cleavage during granzyme B-mediated cytotoxicity

机译：La自身抗原在颗粒酶B介导的细胞毒性作用下裂解后易位至细胞质

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Our recent report demonstrated that apoptosis-specific autoantibodies against granzyme B-induced cleavage fragments of SS-B (La) were found in the sera from patients with primary Sjogren's syndrome. The objective of this study was identified by the intracellular redistribution of La autoantigen during granzyme B-induced apoptosis. We developed green fluorescence protein (GFP)-La and GFP-La Delta 220 (generation of granzyme B-specific cleavage of La protein) fusion proteins. GFP-La protein was localized in the nucleus, whereas the GFP-La Delta 220 protein predominantly existed in the cytoplasm in transformed A293T cells. Nuclear GFP-La protein was translocated to cytoplasm after granzyme B enriched YT cells incubation. La protein in human salivary grand HSG cells is cleaved and translocated from the nucleus to the cytoplasm after YT cell co-cultivation. These results suggest that La protein is cleaved by granzyme B and N-terminal La fragment (27 kD) translocated to the cytoplasm, thus leading to a novel autoantibody production during granzyme B-mediated cytotoxicity. (c) 2007 Elsevier Inc. All rights reserved.
机译：我们的最新报告表明，在患有原发性干燥综合征的患者血清中发现了针对颗粒酶B诱导的SS-B裂解片段的凋亡特异性自身抗体。这项研究的目标是由La自身抗原在颗粒酶B诱导的细胞凋亡期间在细胞内的重新分布确定的。我们开发了绿色荧光蛋白（GFP）-La和GFP-La Delta 220（La蛋白的颗粒酶B特异性裂解）融合蛋白。 GFP-La蛋白位于细胞核中，而GFP-La Delta 220蛋白主要存在于转化的A293T细胞的细胞质中。富含粒酶B的YT细胞孵育后，核GFP-La蛋白转移到细胞质中。 YT细胞共培养后，人类唾液大HSG细胞中的La蛋白被裂解并从细胞核转移到细胞质。这些结果表明La蛋白被颗粒酶B和N端La片段（27 kD）切割到细胞质中裂解，从而导致在颗粒酶B介导的细胞毒性过程中产生新的自身抗体。 （c）2007 Elsevier Inc.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号