• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Subcellular distribution of choline acetyltransferase by immunogold electron microscopy in non-neuronal cells: placenta, airways and murine embryonic stem cells.
【24h】

Subcellular distribution of choline acetyltransferase by immunogold electron microscopy in non-neuronal cells: placenta, airways and murine embryonic stem cells.

机译：免疫金电子显微镜在非神经元细胞中的胆碱乙酰基转移酶的亚细胞分布：胎盘，气道和鼠胚胎干细胞。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Acetylcholine is synthesized in more or less all mammalian cells. However, little is known about the subcellular location of acetylcholine synthesis. Therefore, in the present experiments the subcellular location of the synthesizing enzyme choline acetyltransferase (ChAT) was investigated by anti-ChAT immunogold electron microscopy in human placenta and airways as well as in a murine embryonic stem cell line (CGR8 cell line).Human tissue was obtained as so-called surplus tissue (after delivery/surgical removal because of lung tumor); the CGR8 stem cell line was cultured under standard conditions. For human tissue a monoclonal mouse anti-ChAT antibody (ab) was used and for the CGR8 cell line a polyclonal goat anti-ChAT ab. Immunogold electron microscopy was applied to identify the subcellular location of ChAT.In trophoblast cells (placenta) specific anti-ChAT immunogold deposition was found within the cell membrane, microvilli, and caveolae but also within the cytosol, for example associated with intermediate filaments. In addition, immunogold deposition was identified within mitochondria and the nuclear membrane. In airway epithelial cells anti-ChAT immunogold was found particularly within the apical cell membrane, cilia, submucosa, cytosol and nuclear membrane. Likewise alveolar macrophages showed positive anti-ChAT immunogold within the nucleus, nuclear membrane and granula. Also in the CGR8 cell line positive anti-ChAT immunogold was identified within the cell nucleus and cytosol.The present experiments demonstrate a wide subcellular distribution of ChAT with particular preference of the cell membrane in human epithelial cells.
机译：乙酰胆碱或多或少地在所有哺乳动物细胞中合成。然而，关于乙酰胆碱合成的亚细胞位置知之甚少。因此，在本实验中，通过抗ChAT免疫金电子显微镜在人胎盘和气道以及鼠胚胎干细胞系（CGR8细胞系）中研究了合成酶胆碱乙酰基转移酶（ChAT）的亚细胞位置。作为所谓的多余组织获得（由于肺部肿瘤在分娩/手术切除后）;在标准条件下培养CGR8干细胞系。对于人类组织，使用了单克隆小鼠抗ChAT抗体（ab），对于CGR8细胞系，则使用了多克隆山羊抗ChAT ab。应用免疫金电子显微镜鉴定ChAT的亚细胞位置。在滋养层细胞（胎盘）中，特定的抗ChAT免疫金沉积存在于细胞膜，微绒毛和小窝内，但也存在于胞质溶胶内，例如与中间细丝有关。另外，在线粒体和核膜中鉴定出免疫金沉积。在气道上皮细胞中，特别是在顶端细胞膜，纤毛，粘膜下层，胞质溶胶和核膜中发现了抗ChAT免疫金。同样，肺泡巨噬细胞在细胞核，核膜和颗粒内也显示出阳性的抗ChAT免疫金。同样在CGR8细胞系中，在细胞核和胞质溶胶中鉴定出阳性抗ChAT免疫金。本实验表明，在人上皮细胞中，ChAT具有广泛的亚细胞分布，尤其是细胞膜。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号