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首页> 外文期刊>Life sciences >Derivatives of Dictyostelium differentiation-inducing factors promote mitogen-activated IL-2 production via AP-1 in Jurkat cells.
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Derivatives of Dictyostelium differentiation-inducing factors promote mitogen-activated IL-2 production via AP-1 in Jurkat cells.

机译：Dictyostelium分化诱导因子的衍生物通过Jurkat细胞中的AP-1促进有丝分裂原激活的IL-2产生。

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摘要

AIMS: Differentiation-inducing factors (DIFs) are chlorinated alkylphenones found in the cellular slime mold Dictyostelium discoideum. DIF derivatives exhibit antiproliferative activities and promote glucose consumption in mammalian cells in vitro. Here, we assessed the ability of DIFs to regulate the immune system in a mammalian cell-line and investigated their mechanisms of action. MAIN METHODS: We examined the effects of 30 DIF derivatives on concanavalin A-induced interleukin-2 (IL-2) production (CIIP) in Jurkat T-cells. We also examined the effects of these DIF derivatives on the activity of three transcription factors required for CIIP: namely, activator protein-1 (AP-1), nuclear factor of activated T-cells (NFAT), and nuclear factor kappa B (NFkappaB). KEY FINDINGS: A reporter gene assay suggested that 2 DIF derivatives, termed DIF-1(+1) and DIF-3(3M), significantly promoted CIIP in Jurkat cells, at least in part, by enhancing the activity of AP-1. These 2 DIF derivatives had no significant effect on concanavalin A-induced interferon-gamma production. SIGNIFICANCE: The results suggest that DIF derivatives could be developed as novel drugs for the activation of IL-2 production and resultant stimulation of the immune system.
机译：目的：分化诱导因子（DIF）是在细胞粘液霉菌盘基网柄菌中发现的氯化烷基苯酮。 DIF衍生物在体外具有抗增殖活性并促进哺乳动物细胞中葡萄糖的消耗。在这里，我们评估了DIF调节哺乳动物细胞系中免疫系统的能力，并研究了其作用机理。主要方法：我们检查了30种DIF衍生物对Jurkat T细胞中伴刀豆球蛋白A诱导的白介素2（IL-2）产生（CIIP）的影响。我们还检查了这些DIF衍生物对CIIP所需的三个转录因子的活性的影响：即激活蛋白1（AP-1），活化T细胞的核因子（NFAT）和核因子κB（NFkappB ）。主要发现：一项记者基因检测表明，两种DIF衍生物（称为DIF-1（+1）和DIF-3（3M））至少部分地通过增强AP-1的活性来显着促进Jurkat细胞的CIIP。这两种DIF衍生物对伴刀豆球蛋白A诱导的干扰素-γ产生没有明显影响。意义：结果表明，DIF衍生物可以作为激活IL-2产生并刺激免疫系统的新药而开发。

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