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首页> 外文期刊>Life sciences >QUANTIFICATION OF THE FULL LENGTH LEPTIN RECEPTOR (OB-RB) IN HUMAN BROWN AND WHITE ADIPOSE TISSUE
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QUANTIFICATION OF THE FULL LENGTH LEPTIN RECEPTOR (OB-RB) IN HUMAN BROWN AND WHITE ADIPOSE TISSUE

机译：人体和白色脂肪组织中全长瘦素受体（OB-RB）的定量

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Levels of expression of the leptin receptor (OB-R) splice variants have been studied in human omental white and perirenal brown adipose tissues by reverse transcription-PCR. The level of mRNA expression of the full length form (OB-Rb) was approximately 15% of that of the sum of all splice variants in white or brown adipose tissue. In an attempt to quantify the gene expression of OB-Rb in human white adipose tissue, a quantitative competitive PCR technique was developed, using oligonucleotide primers designed for OB-Rb and an internal standard for a ''MIMIC'' competition strategy. The levels of expression of OB-Rb mRNA in the omental fat of lean and obese patients were compared and no difference could be observed between the two groups. The quantitative RT-PCR technique allows for a fast and accurate measurement of the expression of the OB-Rb mRNA in small tissue samples. [References: 19]
机译：瘦蛋白受体（OB-R）剪接变体的表达水平已通过逆转录PCR在人网膜白色和肾周褐色脂肪组织中进行了研究。全长形式（OB-Rb）的mRNA表达水平约为白色或棕色脂肪组织中所有剪接变体总和的15％。为了量化人白色脂肪组织中OB-Rb的基因表达，开发了一种定量竞争PCR技术，使用为OB-Rb设计的寡核苷酸引物和“ MIMIC”竞争策略的内标。比较了肥胖和肥胖患者网膜脂肪中OB-Rb mRNA的表达水平，两组之间没有差异。定量RT-PCR技术可快速准确地测量小组织样品中OB-Rb mRNA的表达。 [参考：19]

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