• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Effects of absorption enhancers on cytoskeletal actin filaments in Caco-2 cell monolayers.
【24h】

Effects of absorption enhancers on cytoskeletal actin filaments in Caco-2 cell monolayers.

机译：吸收促进剂对Caco-2细胞单层细胞骨架肌动蛋白丝的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Histomorphological changes of actin filaments, intracellular levels of calcium ion, and amount of released lactate dehydrogenase (LDH) were examined, in order to elucidate the mechanism of action of three absorption enhancers, i.e., sodium caprate (Cap-Na), sodium deoxycholate (Deo-Na), and dipotassium glycyrrhizinate (Grz-K), using Caco-2 cell monolayers. The structure of actin filaments in microvilli was slightly modified by 0.5 %(w/v) Grz-K and was significantly changed by 0.2 %(w/v) Cap-Na, 0.05 %(w/v) Deo-Na, and 0.0015 %(w/v) ionomycin. All of the enhancers, except Grz-K, induced significantly histomorphological changes in the actin filaments on the middle depth and basal side of the cells. Furthermore, the altered structure of the actin filaments in the monolayers was restored after removal of the Cap-Na, Grz-K and ionomycin, but not Deo-Na. Intracellular levels of calcium ion increased in the following order: ionomycin = Cap-Na > Deo-Na. However, the intracellular calcium ion levels decreased by treatment with Grz-K. The changes in transepithelial electric resistance (TEER) at the initial stage of treatment with all enhancers correlated with intracellular calcium ion levels. These results suggest that one of the mechanisms by which these agents exert absorption-enhancing activity involves structural alterations in the cytoskeletal actin filaments which are provoked by changes in intracellular calcium ion levels. Only the monolayers which were treated with 0.05 %(w/v) Deo-Na released a significant amount of LDH and irreversibly altered the structure of actin filaments, thus indicating that Deo-Na might affect the actin filaments not only by increasing intracellular calcium ion level but also by other, presently unknown factors.
机译：检查肌动蛋白丝的组织形态学变化，细胞内钙离子水平和释放的乳酸脱氢酶（LDH）的量，以阐明三种吸收增强剂即癸酸钠（Cap-Na），脱氧胆酸钠（ Deo-Na）和甘草酸二钾（Grz-K），使用Caco-2细胞单层膜。微绒毛中肌动蛋白丝的结构被0.5％（w / v）Grz-K轻微修饰，并被0.2％（w / v）Cap-Na，0.05％（w / v）Deo-Na和0.0015显着改变％（w / v）离子霉素。除Grz-K外，所有增强子均在细胞的中层深度和基底侧诱导肌动蛋白丝的组织形态学变化。此外，去除Cap-Na，Grz-K和离子霉素后，单层肌动蛋白丝的结构得以恢复，而Deo-Na则没有。细胞内钙离子水平按以下顺序增加：离子霉素= Cap-Na> Deo-Na。然而，通过用Grz-K处理使细胞内钙离子水平降低。所有增强剂在治疗初期的跨上皮电阻（TEER）的变化与细胞内钙离子水平相关。这些结果表明，这些试剂发挥吸收增强活性的机制之一涉及细胞骨架肌动蛋白丝的结构改变，这是由细胞内钙离子水平的改变引起的。仅用0.05％（w / v）的Deo-Na处理的单层细胞释放出大量的LDH，并且不可逆地改变肌动蛋白丝的结构，因此表明Deo-Na不仅会通过增加细胞内钙离子来影响肌动蛋白丝。水平，但也受其他目前未知的因素影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号