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首页> 外文期刊>Life sciences >Reduced uptake and enhanced release of cadmium in cadmium-resistant metallothionein null fibroblasts
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Reduced uptake and enhanced release of cadmium in cadmium-resistant metallothionein null fibroblasts

机译：耐镉的金属硫蛋白无效成纤维细胞中镉的吸收减少并增强释放

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摘要

Metallothionein (MT) is known to play a predominant role in the protection of cells from cadmium (Cd) toxicity. To investigate other factors involved in Cd resistance, we established Cd-resistant cell lines from simian virus 40-transformed MT null fibroblasts. Cd-resistant MT null cells, Cd-rA7 and Cd-rB5, developed approximately 10-fold resistance to Cd compared to parental cells, but showed no cross-resistance to Zn, Cu, Hg, Ni, As, cisplatin or H_2O_2. Accumulation of Cd in the resistant cells was 13-18% of that of parental cells after treatment with Cd for 24 h. A short-term experiment revealed that the rate of Cd incorporation into the Cd-resistant cells was suppressed, and the rate of Cd release was enhanced in the resistant cells compared with that of parental cells. These results indicate that the altered transport of Cd, slow uptake and rapid release, may confer resistance to Cd on the Cd-resistant cells established from MT null fibroblasts.
机译：已知金属硫蛋白（MT）在保护细胞免受镉（Cd）毒性的过程中起主要作用。为了调查与Cd抗性有关的其他因素，我们从猿猴病毒40转化的MT null成纤维细胞中建立了Cd抗性细胞系。耐CD的MT空细胞Cd-rA7和Cd-rB5与亲代细胞相比，对Cd的耐受性约为10倍，但对Zn，Cu，Hg，Ni，As，顺铂或H_2O_2无交叉抗性。用Cd处理24小时后，耐药细胞中Cd的积累是亲代细胞的13-18％。短期实验显示，与亲代细胞相比，抗性细胞中Cd掺入Cd的速率受到抑制，并且Cd释放速率提高。这些结果表明，改变的Cd转运，缓慢的吸收和快速释放，可能赋予MT空成纤维细胞建立的Cd抗性细胞对Cd的抗性。

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