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首页> 外文期刊>Life sciences >Evidence for an endogenous ouabain-like immunoreactive factor in human newborn plasma coeluted with ouabain on HPLC.
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Evidence for an endogenous ouabain-like immunoreactive factor in human newborn plasma coeluted with ouabain on HPLC.

机译：在HPLC上与哇巴因共洗脱的人新生血浆中内源性哇巴因样免疫反应因子的证据。

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摘要

The identification in human plasma of ouabain as an endogenous digitalis-like factor (EDLF) claimed by Hamlyn et al. has recently been contradicted by two studies which failed to demonstrate endogenous ouabain-like immunoreactivity in HPLC fractions in which exogenous ouabain was eluted. In this paper we report the results obtained on the cross-reactivity with antiouabain antibodies of an EDLF purified by us from human newborn cord plasma. We found that this EDLF coeluted with ouabain on HPLC and cross-reacted both with rabbit anti-ouabain antiserum and with the purified antibodies, which excluded possible interferences due to antibodies directed against non-ouabain portions of the immunogen. Similar but not identical slopes of the ouabain and EDLF displacements curves were observed in all competition ELISA experiments. The inhibitory effect of EDLF on erythrocyte 86Rb uptake was reversed by antiouabain antiserum and antibodies. The concentration of EDLF in newborn plasma, in the four different purifications studied ranged from 30 to 380 pM ouabain equivalents (o.e.) by ELISA and from 100 to 300 pM o.e. by 86Rb uptake. Our data thus support the existence, in human newborn plasma, of a factor with both biological and immunological ouabain-like properties, although not necessarily identical to ouabain.
机译：Hamlyn等人声称在人血浆中哇巴因是一种内源性洋地黄样因子（EDLF）。最近的两项研究相矛盾，这两项研究未能在洗脱外源哇巴因的HPLC馏分中证实内源性哇巴因样免疫反应。在本文中，我们报告了从人类新生儿脐带血浆中纯化的EDLF与抗哇巴因抗体的交叉反应性获得的结果。我们发现该EDLF与哇巴因在HPLC上共洗脱，并与兔抗哇巴因抗血清和纯化的抗体交叉反应，这排除了由于针对免疫原非哇巴因部分的抗体引起的可能干扰。在所有竞争ELISA实验中，观察到的哇巴因和EDLF位移曲线的斜率相似但不相同。 EDLF对红细胞86Rb摄取的抑制作用被抗哇巴因抗血清和抗体逆转。在所研究的四个不同的纯化中，新生儿血浆中EDLF的浓度范围为30至380 pM哇巴因当量（o.e。）（通过ELISA）和100至300 pMo.e。被86Rb吸收。因此，我们的数据支持在人类新生儿血浆中存在具有生物学和免疫学类似哇巴因性质的因子，尽管不一定与哇巴因相同。

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