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首页> 外文期刊>Life sciences >PROLONGED PHORBOL ESTER TREATMENT DOWN-REGULATES PROTEIN KINASE C ISOZYMES AND INCREASES CONTRACTION RATE IN NEONATAL CARDIAC MYOCYTES
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PROLONGED PHORBOL ESTER TREATMENT DOWN-REGULATES PROTEIN KINASE C ISOZYMES AND INCREASES CONTRACTION RATE IN NEONATAL CARDIAC MYOCYTES

机译：长时间的酚酯处理下调蛋白激酶C的同工酶并提高新生心肌细胞的收缩率

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摘要

We have determined the effects of chronic exposure to the protein kinase C (PKC) activating drug 4-beta phorbol 12-myristate-13-acetate (PMA) on PKC isozymes and the rate of spontaneous contraction in neonatal rat cardiac myocytes in culture. Western blot analyses revealed that a two day exposure to 0.1-1 nM PMA increased the total amount of delta PKC, whereas, 100 nM PMA concentrations caused a complete down-regulation of the alpha PKC and an 80 kDa zeta PKC-like protein. In addition, 100 nM PMA treatment for 2 days did not result in complete down-regulation of the beta, delta, and epsilon PKC isozymes in Western blot and immunocytochemical studies. We also found a PKC-mediated enhancement of the rate of contraction in these cells following prolonged exposure to PMA(1-100nM). Our studies suggest that this enhancement of contraction rate may be mediated by the beta,delta, or epsilon PKC isozymes. A better understanding of the role(s) of PKC isozymes in the modulation of cardiac functions may reveal selective targets for therapies of cardiac disorders. [References: 59]
机译：我们已经确定了长期暴露于蛋白激酶C（PKC）活化药物4-βphorbol 12-肉豆蔻酸13-乙酸盐（PMA）对PKC同工酶的影响以及培养的新生大鼠心肌细胞自发收缩的速率。 Western印迹分析显示，暴露于0.1-1 nM PMA两天会增加PKC的总量，而100 nM PMA浓度会导致αPKC和80 kDa zeta PKC样蛋白的完全下调。此外，在蛋白质印迹和免疫细胞化学研究中，100 nM PMA处理2天并未导致β，δ和εPKC同工酶的完全下调。我们还发现延长暴露于PMA（1-100nM）后，这些细胞中PKC介导的收缩率增强。我们的研究表明，收缩率的这种增强可能是由β，δ或epsilon PKC同工酶介导的。更好地了解PKC同工酶在调节心脏功能中的作用，可能揭示出治疗心脏病的选择性靶点。 [参考：59]

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