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首页> 外文期刊>Cell biology international. >Dexamethasone has pro-apoptotic effects on non-activated fresh peripheral blood mononuclear cells.
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Dexamethasone has pro-apoptotic effects on non-activated fresh peripheral blood mononuclear cells.

机译：地塞米松对未活化的新鲜外周血单核细胞具有促凋亡作用。

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Apoptosis is a physiological method of cell death commonly referred to as programmed cell death. However, non-apoptotic programmed cell death, such as autophagy and programmed necrosis, has been characterized by morphological criteria. In view of the human therapeutic use of DEX, and considering that no difference in the number and/or affinity of glucocorticoid receptors in activated and non-activated lymphocytes has been reported, we decided to evaluate the effect of DEX on fresh peripheral blood mononuclear cells (PBMC). Transmission electron microscopy showed that DEX can significantly induce apoptosis in non-activated PBMC. It was also observed by transmission electron microscopy that, independently of DEX treatment, PBMC also died by a process marked by extreme vacuolization and increase in cellular volume; these cells were erroneously classified as viable by flow cytometry using the 7-AAD assay. It is concluded that the DEX pro-apoptotic effect is not restricted to activated PBMC and, therefore, DEX-induced apoptosis could play either homeostatic (activated PBMC) or immunosuppressive (non-activated PBMC) roles.
机译：凋亡是细胞死亡的生理方法，通常称为程序性细胞死亡。但是，非凋亡程序性细胞死亡，例如自噬和程序性坏死，已被形态学标准所表征。考虑到DEX在人类中的治疗用途，并且考虑到活化和非活化淋巴细胞中糖皮质激素受体的数量和/或亲和力均未见报道，我们决定评估DEX对新鲜外周血单核细胞的影响（PBMC）。透射电子显微镜显示，DEX可以在未激活的PBMC中明显诱导细胞凋亡。透射电子显微镜还观察到，与DEX处理无关，PBMC也以极度空泡和细胞体积增加为特征而死亡。使用7-AAD分析通过流式细胞仪将这些细胞错误地归类为有活力。结论是，DEX的促凋亡作用不仅限于活化的PBMC，因此，DEX诱导的凋亡可能起稳态作用（活化的PBMC）或免疫抑制作用（非活化的PBMC）。

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