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首页> 外文期刊>Cell biology international. >Influence of age and sex on the spontaneous DNA damage detected by Micronucleus test and Comet assay in mice peripheral blood cells.
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Influence of age and sex on the spontaneous DNA damage detected by Micronucleus test and Comet assay in mice peripheral blood cells.

机译：年龄和性别对小鼠外周血细胞微核试验和彗星试验检测到的自发性DNA损伤的影响。

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We have investigated the normal variations in basal DNA damage detected by Comet assay in leukocytes and micronucleated erythrocytes (MNE) using the Micronucleus test (MN) in peripheral blood cells from 45 female and male mice from different age groups (newborns, 3.5, 12, and 104 weeks) to clarify age and sex-related changes. Comparison of basal DNA damage detected by Comet assay showed significantly increased values in 104 weeks old mice in relation to the other ages (P < or = 0.01), and newborn mice showed higher values in MNE frequency when compared to all the other groups (P < or = 0.01). A positive correlation was observed between Damage Frequency (r =0.382, P = 0.010) and Damage Index (r = 0.640, P < 0.001) and age. Age was also correlated with the ratio of polychromatic erythrocytesormachromatic erythrocytes (PCE/NCE) (r = -0.473, P = 0.001), and the MNE frequency was positively correlated with the ratio of PCE/NCE (r = 0.454, P = 0.002). These results suggest an age-related slow down of DNA repair efficiency of DNA damage and/or DNA damage accumulation. Furthermore, data on the spontaneous MNE frequency indicate that the reticuloendothelial system matures with age, and there is a close relationship between erythropoiesis and micronucleus induction in erythrocytes. The influence of sex in the parameters analyzed was less clear. In conclusion, age seems to influence in basal DNA damage and should be considered in genotoxicity studies using mice. Finally, comparisons between assays must be made with care when different cells are compared (e.g. leukocytes and erythrocytes), as found with the Comet assay and MN test.
机译：我们使用微核试验（MN）在不同年龄组的45只雌性和雄性小鼠（新生儿，3.5、12，和104周）以明确年龄和性别相关的变化。通过彗星分析检测到的基础DNA损伤的比较显示，与其他年龄相比，104周龄小鼠的值显着增加（P <或= 0.01），而与所有其他组相比，新生小鼠的MNE频率值更高（P <或= 0.01）。损伤频率（r = 0.382，P = 0.010）和损伤指数（r = 0.640，P <0.001）与年龄之间存在正相关。年龄也与多色红细胞/正色红细胞（PCE / NCE）的比例相关（r = -0.473，P = 0.001），而MNE频率与PCE / NCE的比例正相关（r = 0.454，​​P = 0.002）。这些结果表明与年龄有关的DNA损伤和/或DNA损伤积累的DNA修复效率减慢。此外，有关自发MNE频率的数据表明网状内皮系统随着年龄的增长而成熟，并且红细胞生成与红细胞中微核的诱导之间存在密切的关系。性别对所分析参数的影响尚不清楚。总之，年龄似乎会影响基础DNA损伤，在使用小鼠进行的基因毒性研究中应考虑年龄。最后，当比较不同的细胞（例如白细胞和红细胞）时，必须谨慎进行分析之间的比较，如彗星分析和MN测试所发现的。

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