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首页> 外文期刊>Life sciences >Lack of involvement of dopamine and serotonin during the orphanin FQ/Nociceptin (OFQ/N)-induced prolactin secretory response.
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Lack of involvement of dopamine and serotonin during the orphanin FQ/Nociceptin (OFQ/N)-induced prolactin secretory response.

机译：孤儿蛋白FQ / Nociceptin（OFQ / N）诱导的催乳激素分泌反应期间缺乏多巴胺和5-羟色胺的参与。

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The purpose of these studies was to examine possible mechanisms of Orphanin FQ/Nociceptin (OFQ/N)-induced prolactin release. We investigated the involvement of the dopaminergic neurons by quantifying DOPAC:DA levels in the median eminence and neurointermediate lobe following central administration of OFQ/N to female Sprague-Dawley rats. To specifically determine the involvement of the tuberoinfundibular dopaminergic neurons, immunocytochemical studies were conducted to visualize c-fos protein expression in the arcuate nucleus following central administration of OFQ/N. In addition, the role of serotonergic activation was examined in dose response studies using the selective serotonin antagonist ritansarin and the nonselective antagonist metergoline. Finally, the pharmacological specificity of the prolactin response was examined by pretreating animals with [Nphe(1)] NC (1-13)NH(2), a drug reported to antagonize OFQ/N effects. The results of these studies indicate that the increase in prolactin release following central administration of OFQ/N does not inhibit tuberoinfundibular, tuberohypophyseal or periventricular hypophysial dopaminergic neuronal activity at 10 min after drug administration, a time when prolactin levels were significantly elevated. Furthermore, serotonergic activation is not involved since pharmacological blockade of serotonergic receptors did not alter the prolactin secretory response to OFQ/N. NC (1-13)NH(2) did not antagonize the stimulatory effects of OFQ/N on prolactin secretion. The neural effects of OFQ/N on dopaminergic neuronal activity may occur following a different time course than that of the prolactin increase.
机译：这些研究的目的是检查孤儿蛋白FQ / Nociceptin（OFQ / N）诱导催乳激素释放的可能机制。我们通过对雌性Sprague-Dawley大鼠进行OFQ / N集中给药后，通过量化中位隆起和神经中叶中的DOPAC：DA水平来调查多巴胺能神经元的参与。为了明确确定肺底漏斗性多巴胺能神经元的参与，进行了免疫细胞化学研究，以观察在集中给予OFQ / N后弓形核中c-fos蛋白的表达。另外，在剂量反应研究中使用选择性5-羟色胺拮抗剂利坦沙林和非选择性拮抗剂美特古琳对血清素能激活的作用进行了研究。最后，通过用[Nphe（1）] NC（1-13）NH（2）预处理动物来检查催乳素反应的药理学特异性，该药物据报道可拮抗OFQ / N的作用。这些研究的结果表明，在给药后10分钟（即催乳素水平显着升高的时间），集中给予OFQ / N后催乳素释放的增加不会抑制结核漏斗，结核性垂体或脑室周围多巴胺能神经元活性。此外，不涉及血清素能激活，因为血清素能受体的药理学阻断作用不会改变催乳素对OFQ / N的分泌反应。 NC（1-13）NH（2）没有拮抗OFQ / N对催乳素分泌的刺激作用。 OFQ / N对多巴胺能神经元活动的神经作用可能发生在与催乳素增加不同的时间过程之后。

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