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首页> 外文期刊>Life sciences >The effect of N-acetylcysteine in combination with vitamin C on the activity of ornithine decarboxylase of lung carcinoma cells - In vitro
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The effect of N-acetylcysteine in combination with vitamin C on the activity of ornithine decarboxylase of lung carcinoma cells - In vitro

机译：N-乙酰半胱氨酸联合维生素C对肺癌细胞鸟氨酸脱羧酶活性的影响-体外

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Ornithine decarboxylase (ODC) is a marker of lung cancer and is a key enzyme in the synthesis of polyamines, which are necessary for the promotion of the growth of malignant cells. This study assesses the dose-dependent effect of N-acetylcysteine (NAC), a chemopreventive agent, in combination with vitamin C (VC) on the activity of ODC in lung carcinoma cell line, NCI-H82. The cells were subjected to supplementation of NAC and VC both individually and in combination at different dosages for 24 h as well as 48 h. The cells were incubated with radiolabeled L-ornithine (C-14) after the supplementation of NAC and VC individually as well as in combination. A microprocedure was carried out to estimate the activity of ODC in cells after 24 and 48 h of incubation. The activity which was found to be elevated in control cells was decreased significantly on drug supplementation in dose-dependent fashion. The content of nucleic acids also exhibited similar result and [H-3]-thymidine incorporation was also affected by the supplementation. (c) 2006 Elsevier Inc. All rights reserved.
机译：鸟氨酸脱羧酶（ODC）是肺癌的标志物，并且是多胺合成中的关键酶，多胺是促进恶性细胞生长所必需的。这项研究评估了化学预防剂N-乙酰半胱氨酸（NAC）与维生素C（VC）结合对肺癌细胞NCI-H82中ODC活性的剂量依赖性作用。将细胞分别和以不同剂量组合施用NAC和VC补充24小时和48小时。分别或联合补充NAC和VC后，将细胞与放射性标记的L-鸟氨酸（C-14）一起孵育。进行微处理以估计孵育24和48小时后细胞中ODC的活性。发现在对照细胞中升高的活性在以剂量依赖性方式补充药物时显着降低。核酸的含量也表现出相似的结果，并且[H-3]-胸苷的掺入也受到补充的影响。 （c）2006 Elsevier Inc.保留所有权利。

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