• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >EXPRESSION OF G PROTEINS IN HUMAN PLACENTAS FROM PREGNANCIES COMPLICATED BY GESTATIONAL HYPERTENSION
【24h】

EXPRESSION OF G PROTEINS IN HUMAN PLACENTAS FROM PREGNANCIES COMPLICATED BY GESTATIONAL HYPERTENSION

机译：妊娠高血压并发的孕妇血浆中G蛋白的表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Preeclampsia (gestational hypertension) is accompanied by decreased hPL and increased hCG levels in maternal serum. The expression of these peptides as well as the endocrine mechanisms responsible for their regulation in preeclampsia are unknown. We have demonstrated that regulatory GTP-binding proteins (G proteins) are implicated in the modulation of hPL production by placentas from normal pregnancies. In order to extend our knowledge on placental endocrinology, we analyzed in this study the expression of hPL and beta-hCG mRNAs as well as placental G protein a-subunits in pregnancies complicated by gestational hypertension. Western and Northern blot analyses were respectively performed on membrane protein and total mRNA preparations from human placentas of preeclamptic (n = 7) and normal pregnancies (n = 4). The levels of hPL and beta-hCG mRNAs were respectively 108% and 105% of those from normal placentas, suggesting that the altered circulating levels of hPL and beta-hCG are not related to dysfunctional mRNA expression of these peptides. The autoradiographs for G proteins and their mRNAs showed no difference in G protein expression between preeclamptic and normal tissues. Specifically, G alpha i2, G alpha i3, G alpha o, G alpha s, and G alpha q/11 levels reached 87%, 81%, 91%, 99%, and 103% respectively of those from normal placentas. In parallel with the protein levels, their mRNAs expression were respectively 93%, 89%, 113%, 104%, and 94% of normal values for G alpha i2, G alpha i3, G alpha o, G alpha s, and G alpha q/11. These results suggest that neither a change in hPL and beta-hCG expression nor a change in signal transduction machinery is implicated in the altered circulating levels of hPL and beta-hCG in preeclampsia. [References: 29]
机译：子痫前期（妊娠高血压）伴随着母亲血清中hPL降低和hCG水平升高。在先兆子痫中，这些肽的表达以及调节它们的内分泌机制尚不清楚。我们已证明，调节性GTP结合蛋白（G蛋白）与正常妊娠胎盘产生的hPL产生有关。为了扩展我们对胎盘内分泌学的了解，我们在这项研究中分析了hPL和β-hCGmRNA以及胎盘G蛋白a亚基在妊娠高血压合并妊娠中的表达。分别对先兆子痫（n = 7）和正常妊娠（n = 4）的人胎盘的膜蛋白和总mRNA制剂进行了Western和Northern blot分析。 hPL和β-hCGmRNA的水平分别为正常胎盘的108％和105％，这表明hPL和β-hCG循环水平的改变与这些肽的功能异常mRNA表达无关。 G蛋白及其mRNA的放射自显影照片显示先兆子痫和正常组织之间G蛋白的表达没有差异。具体而言，G alpha i2，G alpha i3，G alpha o，G alpha s和G alpha q / 11的水平分别达到正常胎盘的87％，81％，91％，99％和103％。与蛋白质水平平行，它们的mRNA表达分别为G alpha i2，G alpha i3，G alpha o，G alpha s和G alpha正常值的93％，89％，113％，104％和94％。 q / 11。这些结果表明，先兆子痫中hPL和β-hCG的循环水平改变均不涉及hPL和β-hCG表达的变化或信号转导机制的变化。 [参考：29]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号